Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Kyonggi-Do 16227, Republic of Korea.
Department of Biological Science, College of Science and Engineering, Sangji University, Wonju, Republic of Korea.
Int J Syst Evol Microbiol. 2020 Apr;70(4):2537-2553. doi: 10.1099/ijsem.0.004071. Epub 2020 Mar 11.
Fifteen isolates of the genus were obtained from Arctic soil samples. All isolates were Gram-stain-negative and rod-shaped. Cells were strictly aerobic, psychrotolerant and grew optimally at 15-20 °C. Phylogenetic analysis based on 16S rRNA gene sequences revealed that all the isolated strains formed a lineage within the family and clustered as members of the genus . The sole respiratory quinone was MK-7 and the major polar lipid was phosphatidylethanolamine. The major cellular fatty acids were summed feature 3 (iso-C2-OH/Cω7ω6), iso-C and iso-C 3-OH. The DNA G+C content of the novel strains was 33.9-41.8 mol%. In addition, the average nucleotide identity and DNA-DNA hybridization relatedness values between the novel type strains and phylogenetically related type strains were below the threshold values used for species delineation. Based on genomic, chemotaxonomic, phenotypic, phylogenetic and phylogenomic analyses, the isolated strains represent novel species in the genus , for which the names sp. nov. (type strain AR-2-6=KEMB 9005-717=KACC 19998=NBRC 113826), sp. nov. (type strain AR-3-17=KEMB 9005-718=KACC 19999=NBRC 113827), sp. nov. (type strain RP-1-13=KEMB 9005-720=KACC 21147=NBRC 113829), sp. nov. (type strain RP-1-14=KEMB 9005-721=KACC 21148=NBRC 113830), sp. nov. (type strain RP-3-8=KEMB 9005-724=KACC 21152=NBRC 113833), sp. nov. (type strain RP-3-11=KEMB 9005-725=KACC 21153=NBRC 113927), sp. nov. (type strain RP-3-15=KEMB 9005-726=KACC 21154=NBRC 113834), sp. nov. (type strain RP-3-21=KEMB 9005-728=KACC 21156=NBRC 113835) and sp. nov. (type strain RP-3-22=KEMB 9005-729=KACC 21157=NBRC 113836) are proposed.
从北极土壤样本中获得了 15 株 属的分离株。所有分离株均为革兰氏阴性、杆状。细胞为严格需氧、嗜冷，最适生长温度为 15-20°C。基于 16S rRNA 基因序列的系统发育分析表明，所有分离株均形成了一个家族内的谱系，并聚类为属的成员。唯一的呼吸醌为 MK-7，主要极性脂为磷脂酰乙醇胺。主要的细胞脂肪酸为总和特征 3（iso-C2-OH/Cω7ω6）、iso-C 和 iso-C 3-OH。新型菌株的 DNA G+C 含量为 33.9-41.8 mol%。此外，新型菌株与系统发育相关的模式菌株之间的平均核苷酸同一性和 DNA-DNA 杂交相关性值低于用于物种划分的阈值。基于基因组、化学分类学、表型、系统发育和系统基因组分析，分离株代表了属中的新型物种，建议将其命名为 sp. nov.（模式菌株 AR-2-6=KEMB 9005-717=KACC 19998=NBRC 113826）、 sp. nov.（模式菌株 AR-3-17=KEMB 9005-718=KACC 19999=NBRC 113827）、 sp. nov.（模式菌株 RP-1-13=KEMB 9005-720=KACC 21147=NBRC 113829）、 sp. nov.（模式菌株 RP-1-14=KEMB 9005-721=KACC 21148=NBRC 113830）、 sp. nov.（模式菌株 RP-3-8=KEMB 9005-724=KACC 21152=NBRC 113833）、 sp. nov.（模式菌株 RP-3-11=KEMB 9005-725=KACC 21153=NBRC 113927）、 sp. nov.（模式菌株 RP-3-15=KEMB 9005-726=KACC 21154=NBRC 113834）、 sp. nov.（模式菌株 RP-3-21=KEMB 9005-728=KACC 21156=NBRC 113835）和 sp. nov.（模式菌株 RP-3-22=KEMB 9005-729=KACC 21157=NBRC 113836）。
