Department of Animal Sciences, Arak Branch, Islamic Azad University, Arak, Iran.
Department of Animal Sciences, Arak Branch, Islamic Azad University, Arak, Iran.
Poult Sci. 2021 Sep;100(9):101289. doi: 10.1016/j.psj.2021.101289. Epub 2021 May 27.
This study evaluates the effects of pumpkin seed oil and sunflower oil along with vitamin E on the reproductive characteristics of aged roosters. Thirty Ross breeder roosters (45-wk-old) were assigned into 6 groups (5 birds/group) with the following diets: 1) control group (basal diet), 2) basal diet with 2% pumpkin seed oil as omega-3 fatty acid (PSO group), 3) basal diet with 2% sunflower oil as omega-6 fatty acid (SFO group), 4) basal diet with 200 mg/kg vitamin E (Control + vitE group), 5) basal diet and 2% pumpkin seed oil along with 200 mg/kg vitamin E (PSO + vitE group) and 6) basal diet and 2% sunflower oil along with 200 mg/kg vitamin E (SFO + vitE group). Roosters were fed on the experimental diets for 60 d and different characteristics of sperm characteristics including routine semen analysis and several sperm functional tests in every 20 d were examined. Reproductive hormones were also evaluated in 0 d and at the end of the trial. Semen volume and morphology were not affected by any of the diets. The roosters fed with pumpkin seed oil + vitE showed the higher percentage of sperm concentration, total motility, progressive motility, viability and membrane integrity and the lower lipid peroxidation (P ≤ 0.05). The group 5 (PSO + vitE) had numerically the lowest sperm with fragmented DNA (DNA Fr) at 0 of the experiment and sperm with non-fragmented DNA (DNA Fr) was lowest in group 6 (SFO + vitE) on da 40 the experiment. Testosterone level was not affected by the experimental diets (P > 0.05), however other hormones (LH and FSH) were affected. Based on the results, the supplementation of aged roosters' diet with pumpkin seed oil + vitE improves reproductive performance which can be an appropriate strategy to preserve the reproductive performance of aged roosters.
本研究评估了南瓜籽油和葵花籽油以及维生素 E 对老龄公鸡生殖特性的影响。将 30 只罗斯育雏公鸡（45 周龄）分为 6 组（每组 5 只），给予以下饮食：1）对照组（基础饮食），2）基础饮食添加 2%南瓜籽油作为 ω-3 脂肪酸（PSO 组），3）基础饮食添加 2%葵花籽油作为 ω-6 脂肪酸（SFO 组），4）基础饮食添加 200mg/kg 维生素 E（Control + vitE 组），5）基础饮食添加 2%南瓜籽油和 200mg/kg 维生素 E（PSO + vitE 组）和 6）基础饮食添加 2%葵花籽油和 200mg/kg 维生素 E（SFO + vitE 组）。公鸡在实验饮食中喂养 60 天，每隔 20 天检查精子特征的常规精液分析和几项精子功能测试。在 0 天和试验结束时还评估了生殖激素。任何饮食都不会影响精液量和形态。饲喂南瓜籽油 + 维生素 E 的公鸡精子浓度、总活力、前向运动活力、活力和膜完整性更高，脂质过氧化水平更低（P≤0.05）。在实验的 0 天，第 5 组（PSO + vitE）的精子碎片化 DNA（DNA Fr）比例最低，第 6 组（SFO + vitE）的精子非碎片化 DNA（DNA Fr）最低。实验期间，睾酮水平不受实验饮食的影响（P>0.05），但其他激素（LH 和 FSH）受到影响。基于结果，在老龄公鸡的饮食中添加南瓜籽油+维生素 E 可提高繁殖性能，这是维持老龄公鸡繁殖性能的一种合适策略。
