Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, PO Box 14115-336, Iran.
Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, PO Box 14115-336, Iran.
Poult Sci. 2020 Feb;99(2):708-718. doi: 10.1016/j.psj.2019.12.031. Epub 2020 Jan 24.
This study was conducted to evaluate the effects of dietary polyunsaturated fatty acids (PUFA) sources and rosemary leaves powder (RLP) on the semen quality, fatty acid analysis, and some reproductive hormones of senescent broiler breeder roosters. Thirty-five 45-wk-old Ross breeder roosters were randomly divided into 7 groups (5 birds/group), and received following treatments including control group (basal diet), fish oil (2%), corn oil (2%), an equal (50:50%) proportion of fish oil and corn oil (50:50%), fish oil (2%) with 5 g/kg capsulated RLP, corn oil (2%) with 5 g/kg capsulated RLP, and an equal (50:50) proportion of fish oil and corn oil (50:50%) with 5 g/kg capsulated RLP of diet for 60 D, during which time their seminal characteristics were evaluated every 20 D. At the end of the trial (on day 60), semen samples were tested for determination of sperm fatty acid analysis, lipid peroxidation, and some reproductive hormones. Results showed that feeding fish oil and fish/corn oil with RLP was associated with an increase in docosahexaenoic acid (C22:6n-3) and docosatetraenoic acid (C22:4n-6) in sperm. The fish oil diet increased the proportion of n-3 fatty acids in sperm, and as a consequence, the (n-6)/(n-3) ratio also decreased (P < 0.05). RLP (5 g/kg) to the fish and fish/corn-oil (50:50%)-based diet resulted in improvement in sperm concentration, total motility (%), sperm progressive motility (%), membrane integrity, and viability in terms 0 to 60 day trial (P < 0.05). Diets and age interacted to positively affect sperm concentration and sperm membrane integrity. Also this herbal antioxidant decreased the seminal content of malondialdehyde (MDA) significantly (P < 0.05). Testosterone and LH serum levels of reproductive hormones were significantly higher in fish and fish/corn-oil with RPL (50:50%)-based diet than other groups (P < 0.05). It can be concluded that RLP as an antioxidant could remarkably improve the effects of n-3 and n-3/n-6 PUFA on sperm characteristics, seminal MDA, and hormones levels in aged breeder roosters. The susceptibility of semen to lipid peroxidation was increased in chickens fed without RLP. Future studies are needed to disclose the causal mechanisms involved.
本研究旨在评估多不饱和脂肪酸（PUFA）来源和迷迭香叶粉（RLP）对衰老肉鸡种公鸡精液质量、脂肪酸分析和一些生殖激素的影响。35 只 45 周龄罗斯种公鸡随机分为 7 组（每组 5 只），分别接受以下处理：对照组（基础日粮）、鱼油（2%）、玉米油（2%）、鱼油（2%）和玉米油（2%）（50:50%）、鱼油（2%）加 5 g/kg 包被 RLP、玉米油（2%）加 5 g/kg 包被 RLP 和日粮中（50:50%）的鱼油和玉米油（50:50%）加 5 g/kg 包被 RLP，共 60 天，每隔 20 天评估一次精液特性。试验结束时（第 60 天），检测精液样本以确定精子脂肪酸分析、脂质过氧化和一些生殖激素。结果表明，饲喂鱼油和添加 RLP 的鱼油/玉米油可增加精子中二十二碳六烯酸（C22:6n-3）和二十二碳四烯酸（C22:4n-6）的含量。鱼油饮食增加了精子中 n-3 脂肪酸的比例，因此（n-6）/（n-3）的比例也降低（P < 0.05）。RLP（5 g/kg）添加到鱼油和鱼油/玉米油（50:50%）日粮中可改善精子浓度、总活力（%）、精子前向运动活力（%）、膜完整性和活力，60 天试验期间（P < 0.05）。日粮和年龄的相互作用对精子浓度和精子膜完整性有积极影响。此外，这种植物抗氧化剂显著降低了精液中丙二醛（MDA）的含量（P < 0.05）。生殖激素血清中睾酮和 LH 水平在添加 RLP 的鱼油和鱼油/玉米油（50:50%）日粮组显著高于其他组（P < 0.05）。综上所述，RLP 作为一种抗氧化剂，可显著改善 n-3 和 n-3/n-6 PUFA 对老龄种公鸡精子特性、精液 MDA 和激素水平的影响。未添加 RLP 的鸡群精液对脂质过氧化的敏感性增加。需要进一步的研究来揭示涉及的因果机制。
