Hamitouche Fella, Armengaud Jean, Dedieu Luc, Duport Catherine
Biology Department, Campus Jean-Henri Fabre, Avignon University, INRAE, UMR SQPOV, CEDEX 09, 84911 Avignon, France.
Département Médicaments et Technologies pour la Santé (DMTS), Université Paris-Saclay, CEA, INRAE, SPI, 30200 Bagnols-sur-Cèze, France.
Int J Mol Sci. 2021 Jul 14;22(14):7550. doi: 10.3390/ijms22147550.
At the end of exponential growth, aerobic bacteria have to cope with the accumulation of endogenous reactive oxygen species (ROS). One of the main targets of these ROS is cysteine residues in proteins. This study uses liquid chromatography coupled to high-resolution tandem mass spectrometry to detect significant changes in protein abundance and thiol status for cysteine-containing proteins from during aerobic exponential growth. The proteomic profiles of cultures at early-, middle-, and late-exponential growth phases reveals that (i) enrichment in proteins dedicated to fighting ROS as growth progressed, (ii) a decrease in both overall proteome cysteine content and thiol proteome redox status, and (iii) changes to the reduced thiol status of some key proteins, such as the transition state transcriptional regulator AbrB. Taken together, our data indicate that growth under oxic conditions requires increased allocation of protein resources to attenuate the negative effects of ROS. Our data also provide a strong basis to understand the response mechanisms used by to deal with endogenous oxidative stress.
在指数生长末期，需氧细菌必须应对内源性活性氧（ROS）的积累。这些ROS的主要靶点之一是蛋白质中的半胱氨酸残基。本研究使用液相色谱与高分辨率串联质谱联用技术，来检测在有氧指数生长期间含半胱氨酸蛋白质的蛋白质丰度和硫醇状态的显著变化。处于指数生长早期、中期和晚期的培养物的蛋白质组学图谱显示：（i）随着生长进程，致力于对抗ROS的蛋白质富集；（ii）整体蛋白质组半胱氨酸含量和硫醇蛋白质组氧化还原状态均下降；（iii）一些关键蛋白质（如过渡态转录调节因子AbrB）的还原型硫醇状态发生变化。综上所述，我们的数据表明，在有氧条件下生长需要增加蛋白质资源的分配，以减轻ROS的负面影响。我们的数据也为理解[具体细菌名称未给出]用于应对内源性氧化应激的反应机制提供了有力依据。
