Department of Biochemistry, Faculty of Dental Medicine, Carol Davila University of Medicine and Pharmacy, 8 Eroilor Sanitari Blvd, Sector 5, 050474 Bucharest, Romania.
Department of Complete Denture, Faculty of Dental Medicine, Carol Davila University of Medicine and Pharmacy, 8 Eroilor Sanitari Blvd, Sector 5, 050474 Bucharest, Romania.
Molecules. 2021 Jul 7;26(14):4129. doi: 10.3390/molecules26144129.
A wide range of mediators are released from the pulp tissue because of bacterial invasion which causes inflammation. Interleukins (ILs) and matrix metalloproteinases (MMPs) have a leading role in initiating and spreading of inflammation because of their synergic action. Biomarkers such as ILs and MMPs can be identified via several methods, establishing the inflammatory response of the dental pulp. The aim of this systematic review is to evaluate the levels of ILs and/or MMPs in human dental pulp. PubMed, OVID, Cochrane, Scopus, Web of Science and Wiley online library databases were searched for original clinical studies. After applying inclusion and exclusion criteria, a quality assessment of studies was performed based on a modified Newcastle-Ottawa scale. In the review were included articles that evaluated the presence of ILs and/or MMPs in pulp tissue using enzyme-linked immunosorbent assay (ELISA) or western blot or multiplex assay. Six articles were included in the present synthesis. Although various diagnostic methods were used, statistically significant higher levels of ILs and/or MMPs were mostly found in the experimental groups compared to healthy pulp samples. The biomarkers studied can be a promising tool to evaluate pulp tissue health or even in pulpitis treatment.
由于细菌入侵导致炎症，牙髓组织会释放出多种介质。白细胞介素 (ILs) 和基质金属蛋白酶 (MMPs) 由于协同作用，在炎症的启动和扩散中起主要作用。可以通过多种方法识别生物标志物，如 ILs 和 MMPs，以确定牙髓的炎症反应。本系统评价旨在评估人牙髓中 ILs 和/或 MMPs 的水平。通过 PubMed、OVID、Cochrane、Scopus、Web of Science 和 Wiley online library 数据库搜索原始临床研究。在应用纳入和排除标准后，根据改良的 Newcastle-Ottawa 量表对研究进行质量评估。在本次综合研究中，共纳入了六篇评估使用酶联免疫吸附试验 (ELISA) 或 Western blot 或多重分析检测牙髓组织中 ILs 和/或 MMPs 存在的文章。尽管使用了各种诊断方法，但与健康牙髓样本相比，实验组中大多数情况下 ILs 和/或 MMPs 的水平明显更高。研究中的生物标志物可能是评估牙髓组织健康甚至牙髓治疗的有前途的工具。
