干旱胁迫条件下种植的意大利古老番茄品种果皮提取物对血管相关功能障碍的影响。

Impact of Peels Extracts from an Italian Ancient Tomato Variety Grown under Drought Stress Conditions on Vascular Related Dysfunction.

机构信息

Cardiovascular Research Laboratory, Department of Surgical, Medical and Molecular Pathology and Critical Care Medicine, University of Pisa, 56100 Pisa, Italy.

Department of Life Sciences, University of Siena, Via P.A. Mattioli 4, 53100 Siena, Italy.

出版信息

Molecules. 2021 Jul 15;26(14):4289. doi: 10.3390/molecules26144289.

DOI:10.3390/molecules26144289

PMID:34299564

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8307906/

Abstract

BACKGROUND

Tomato by-products contain a great variety of biologically active substances and represent a significant source of natural antioxidant supplements of the human diet. The aim of the work was to compare the antioxidant properties of a by-product from an ancient Tuscan tomato variety, Rosso di Pitigliano (RED), obtained by growing plants in normal conditions (-Ctr) or in drought stress conditions (-Ds) for their beneficial effects on vascular related dysfunction.

METHODS

The antioxidant activity and total polyphenol content (TPC) were measured. The identification of bioactive compounds of tomato peel was performed by HPLC. HUVEC were pre-treated with different TPC of RED-Ctr or RED-Ds, then stressed with HO. Cell viability, ROS production and CAT, SOD and GPx activities were evaluated. Permeation of antioxidant molecules contained in RED across excised rat intestine was also studied.

RESULTS

RED-Ds tomato peel extract possessed higher TPC than compared to RED-Ctr (361.32 ± 7.204 mg vs. 152.46 ± 1.568 mg GAE/100 g fresh weight). All extracts were non-cytotoxic. Two hour pre-treatment with 5 µg GAE/mL from RED-Ctr or RED-Ds showed protection from HO-induced oxidative stress and significantly reduced ROS production raising SOD and CAT activity (* < 0.05 and ** < 0.005 vs. HO, respectively). The permeation of antioxidant molecules contained in RED-Ctr or RED-Ds across excised rat intestine was high with non-significant difference between the two RED types (41.9 ± 9.6% vs. 26.6 ± 7.8%).

CONCLUSIONS

RED-Ds tomato peel extract represents a good source of bioactive molecules, which protects HUVECs from oxidative stress at low concentration.

摘要

背景

番茄副产物含有大量具有生物活性的物质，是人类饮食中天然抗氧化补充剂的重要来源。本工作旨在比较通过在正常条件下（-Ctr）或干旱胁迫条件下（-Ds）种植植物获得的托斯卡纳古老番茄品种 Rosso di Pitigliano（RED）副产物的抗氧化特性，以观察其对血管相关功能障碍的有益影响。

方法

测定抗氧化活性和总多酚含量（TPC）。采用高效液相色谱法鉴定番茄皮中的生物活性化合物。用不同 TPC 的 RED-Ctr 或 RED-Ds 预处理 HUVEC，然后用 HO 应激。评估细胞活力、ROS 产生以及 CAT、SOD 和 GPx 活性。还研究了 RED 中抗氧化分子在离体大鼠肠中的渗透。

结果

与 RED-Ctr（361.32 ± 7.204 mg 比 152.46 ± 1.568 mg GAE/100 g 鲜重）相比，RED-Ds 番茄皮提取物具有更高的 TPC。所有提取物均无细胞毒性。用 5 µg GAE/mL 的 RED-Ctr 或 RED-Ds 预处理 2 小时可预防 HO 诱导的氧化应激，并显著降低 ROS 产生，提高 SOD 和 CAT 活性（* < 0.05 和 ** < 0.005 与 HO 相比，分别）。RED-Ctr 或 RED-Ds 中抗氧化分子的渗透穿过离体大鼠肠很高，两种 RED 类型之间无显著差异（41.9 ± 9.6% 比 26.6 ± 7.8%）。