干旱胁迫条件下生长的番茄皮提取物对肌少症模型的影响。

Effect of Tomato Peel Extract Grown under Drought Stress Condition in a Sarcopenia Model.

机构信息

Department of Surgical, Medical and Molecular Pathology and Critical Care Medicine, University of Pisa, 56100 Pisa, Italy.

Department of Life Sciences, University of Siena, Via P.A. Mattioli 4, 53100 Siena, Italy.

出版信息

Molecules. 2022 Apr 15;27(8):2563. doi: 10.3390/molecules27082563.

Tomatoes and their derivates represent an important source of natural biologically active components. The present study aims to investigate the protective effect of tomato peel extracts, grown in normal (RED-Ctr) or in drought stress (RED-Ds) conditions, on an experimental model of sarcopenia. The phenolic profile and total polyphenols content (TPC) of RED-Ctr and RED-Ds were determined by Ultra High-Performance Liquid Chromatography (UHPLC) analyses coupled to electrospray ionization high-resolution mass spectrometry (ESI-HR-MS). Human skeletal muscle myoblasts (HSMM) were differentiated in myotubes, and sarcopenia was induced by dexamethasone (DEXA) treatment. Differentiation and sarcopenia were evaluated by both real-time PCR and immunofluorescent techniques. Data show that myosin heavy chain 2 (MYH2), troponin T (TNNT1), and miogenin (MYOG) were expressed in differentiated myotubes. 5 μg Gallic Acid Equivalent (GAE/mL) of TPC from RED-Ds extract significantly reduced muscle atrophy induced by DEXA. Moreover, Forkhead BoxO1 (FOXO1) expression, involved in cell atrophy, was significantly decreased by RED-Ds extract. The protective effect of tomato peel extracts depended on their qualitative polyphenolic composition, resulting effectively in the in vitro model of sarcopenia.

番茄及其衍生物是天然生物活性成分的重要来源。本研究旨在探讨在正常（RED-Ctr）或干旱胁迫（RED-Ds）条件下生长的番茄皮提取物对肌少症实验模型的保护作用。采用超高效液相色谱（UHPLC）分析结合电喷雾电离高分辨质谱（ESI-HR-MS）测定 RED-Ctr 和 RED-Ds 的酚类成分和总多酚含量（TPC）。将人骨骼肌成肌细胞（HSMM）分化为肌管，并通过地塞米松（DEXA）处理诱导肌少症。通过实时 PCR 和免疫荧光技术评估分化和肌少症。结果表明，肌球蛋白重链 2（MYH2）、肌钙蛋白 T（TNNT1）和肌生成素（MYOG）在分化的肌管中表达。RED-Ds 提取物中 5μg 没食子酸当量（GAE/mL）的 TPC 可显著减轻 DEXA 诱导的肌肉萎缩。此外，参与细胞萎缩的叉头框 O1（FOXO1）表达也被 RED-Ds 提取物显著降低。番茄皮提取物的保护作用取决于其定性的多酚组成，可有效改善体外肌少症模型。