可可副产品与樱桃多酚提取物的抗氧化作用：一项对比研究。

Antioxidant Effect of Cocoa By-Product and Cherry Polyphenol Extracts: A Comparative Study.

作者信息

Felice Francesca, Fabiano Angela, De Leo Marinella, Piras Anna Maria, Beconcini Denise, Cesare Maria Michela, Braca Alessandra, Zambito Ylenia, Di Stefano Rossella

机构信息

Cardiovascular Research Laboratory, Department of Surgical, Medical and Molecular Pathology and Critical Care Medicine, University of Pisa, 56100 Pisa, Italy.

Department of Pharmacy, University of Pisa, 56126 Pisa, Italy.

出版信息

Antioxidants (Basel). 2020 Feb 3;9(2):132. doi: 10.3390/antiox9020132.

BACKGROUND

Recent studies have highlighted the importance of cherry and cocoa extracts consumption to protect cells from oxidative stress, paying particular attention to cocoa by-products. This study aims to investigate the protective effect of cocoa husk extract (CHE) and cherry extracts (CE) against ROS-induced oxidative stress in Human Umbilical Vein Endothelial Cells (HUVECs).

METHODS

CE and CHE had antioxidant activity characterized by total polyphenols content (TPC). HUVECs were treated for 2 h and 24 h with increasing TPC concentrations of CE and CHE (5-10-25-50-100 µg Gallic Acid Equivalent (GAE)/mL) and then with HO for 1 h. Cell viability and ROS production were evaluated. CE and CHE polyphenols permeability on excised rat intestine were also studied.

RESULTS

CE and CHE showed a similar antioxidant activity (2.5 ± 0.01 mmol Fe/100 g FW (fresh weight) and 2.19 ± 0.09 mmol Fe/100 g FW, respectively, > 0.05) whereas CHE had a higher TPC (7105.0 ± 96.9 mg GAE/100 g FW) than CE (402.5 ± 8.4 mg GAE/100 g), < 0.05. The in vitro viability assay showed that both extracts were non-cytotoxic. CHE resulted in protection against ROS at lower concentrations than CE. CHE showed a 2-fold higher apparent permeability compared to CE.

CONCLUSIONS

CHE represents a high-value antioxidant source, which is interesting for the food and pharmaceutical industries.

背景

最近的研究强调了食用樱桃和可可提取物对保护细胞免受氧化应激的重要性，尤其关注可可副产品。本研究旨在调查可可壳提取物（CHE）和樱桃提取物（CE）对人脐静脉内皮细胞（HUVECs）中活性氧诱导的氧化应激的保护作用。

方法

通过总多酚含量（TPC）表征CE和CHE的抗氧化活性。用CE和CHE的TPC浓度递增（5-10-25-50-100微克没食子酸当量（GAE）/毫升）处理HUVECs 2小时和24小时，然后用HO处理1小时。评估细胞活力和活性氧生成。还研究了CE和CHE多酚在离体大鼠肠道上的通透性。

结果

CE和CHE表现出相似的抗氧化活性（分别为2.5±0.01毫摩尔铁/100克鲜重（FW）和2.19±0.09毫摩尔铁/100克FW，>0.05），而CHE的TPC（7105.0±96.9毫克GAE/100克FW）高于CE（402.5±8.4毫克GAE/100克），<0.05。体外活力测定表明两种提取物均无细胞毒性。CHE在比CE更低的浓度下就能对活性氧起到保护作用。与CE相比，CHE的表观通透性高2倍。