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用于将质粒DNA递送至胶质母细胞瘤的柠檬酸盐包覆磁性聚乙烯亚胺复合材料

Citrate-Coated Magnetic Polyethyleneimine Composites for Plasmid DNA Delivery into Glioblastoma.

作者信息

Leung Ken Cham-Fai, Sham Kathy W Y, Lai Josie M Y, Wang Yi-Xiang J, Wong Chi-Hin, Cheng Christopher H K

机构信息

State Key Laboratory of Environmental and Biological Analysis, Department of Chemistry, The Hong Kong Baptist University, Kowloon Tong, KLN, Hong Kong, China.

School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China.

出版信息

Polymers (Basel). 2021 Jul 6;13(14):2228. doi: 10.3390/polym13142228.

DOI:10.3390/polym13142228
PMID:34300986
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8309231/
Abstract

Several ternary composites that are based on branched polyethyleneimine (bPEI 25 kDa, polydispersity 2.5, 0.1 or 0.2 ng), citrate-coated ultrasmall superparamagnetic iron oxide nanoparticles (citrate-NPs, 8-10 nm, 0.1, 1.0, or 2.5 µg), and reporter circular plasmid DNA pEGFP-C1 or pRL-CMV (pDNA 0.5 µg) were studied for optimization of the best composite for transfection into glioblastoma U87MG or U138MG cells. The efficiency in terms of citrate-NP and plasmid DNA gene delivery with the ternary composites could be altered by tuning the bPEI/citrate-NP ratios in the polymer composites, which were characterized by Prussian blue staining, in vitro magnetic resonance imaging as well as green fluorescence protein and luciferase expression. Among the composites prepared, 0.2 ng bPEI/0.5 μg pDNA/1.0 µg citrate-NP ternary composite possessed the best cellular uptake efficiency. Composite comprising 0.1 ng bPEI/0.5 μg pDNA/0.1 μg citrate-NP gave the optimal efficiency for the cellular uptake of the two plasmid DNAs to the nucleus. The best working bPEI concentration range should not exceed 0.2 ng/well to achieve a relatively low cytotoxicity.

摘要

研究了几种基于支化聚乙烯亚胺（25 kDa，多分散性2.5，0.1或0.2 ng）、柠檬酸盐包覆的超小超顺磁性氧化铁纳米颗粒（柠檬酸盐-NPs，8-10 nm，0.1、1.0或2.5 μg）以及报告基因环状质粒DNA pEGFP-C1或pRL-CMV（pDNA 0.5 μg）的三元复合材料，以优化用于转染成胶质细胞瘤U87MG或U138MG细胞的最佳复合材料。通过调整聚合物复合材料中bPEI/柠檬酸盐-NP的比例，可以改变三元复合材料在柠檬酸盐-NP和质粒DNA基因递送方面的效率，这些复合材料通过普鲁士蓝染色、体外磁共振成像以及绿色荧光蛋白和荧光素酶表达进行表征。在所制备的复合材料中，0.2 ng bPEI/0.5 μg pDNA/1.0 μg柠檬酸盐-NP三元复合材料具有最佳的细胞摄取效率。包含0.1 ng bPEI/0.5 μg pDNA/0.1 μg柠檬酸盐-NP的复合材料在将两种质粒DNA摄取到细胞核方面具有最佳效率。为了实现相对较低的细胞毒性，最佳工作bPEI浓度范围不应超过0.2 ng/孔。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace6/8309231/0c3e0b0a399f/polymers-13-02228-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace6/8309231/5b273451c11e/polymers-13-02228-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace6/8309231/6df49beadcaa/polymers-13-02228-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace6/8309231/70196b990868/polymers-13-02228-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace6/8309231/88c9505071a1/polymers-13-02228-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace6/8309231/0c3e0b0a399f/polymers-13-02228-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace6/8309231/5b273451c11e/polymers-13-02228-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace6/8309231/6df49beadcaa/polymers-13-02228-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace6/8309231/70196b990868/polymers-13-02228-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace6/8309231/88c9505071a1/polymers-13-02228-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ace6/8309231/0c3e0b0a399f/polymers-13-02228-g004.jpg

相似文献

[1]
Citrate-Coated Magnetic Polyethyleneimine Composites for Plasmid DNA Delivery into Glioblastoma.

Polymers (Basel). 2021-7-6

[2]
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Quant Imaging Med Surg. 2015-6

[3]
Enhanced cellular uptake and gene delivery of glioblastoma with deferoxamine-coated nanoparticle/plasmid DNA/branched polyethylenimine composites.

Chem Commun (Camb). 2012-11-28

[4]
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Biomaterials. 2009-9-26

[5]
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[6]
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Quant Imaging Med Surg. 2013-12

[7]
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J Gene Med. 2004-1

[8]
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Beilstein J Nanotechnol. 2022-1-18

[9]
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[10]
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引用本文的文献

[1]
Effect of Citrate- and Gold-Stabilized Superparamagnetic Iron Oxide Nanoparticles on Head and Neck Tumor Cell Lines during Combination Therapy with Ionizing Radiation.

Bioengineering (Basel). 2022-12-15

本文引用的文献

[1]
Non-cationic Material Design for Nucleic Acid Delivery.

Adv Ther (Weinh). 2020-3

[2]
Gold Nanoparticles Functionalized with Angiogenin for Wound Care Application.

Nanomaterials (Basel). 2021-1-14

[3]
Polymer Based Bioadhesive Biomaterials for Medical Application-A Perspective of Redefining Healthcare System Management.

Polymers (Basel). 2020-12-16

[4]
A Dual Receptor Targeting- and BBB Penetrating- Peptide Functionalized Polyethyleneimine Nanocomplex for Secretory Endostatin Gene Delivery to Malignant Glioma.

Int J Nanomedicine. 2020-11-12

[5]
MALDI-MS Imaging Analysis of Noninflammatory Type III Rotaxane Dendrimers.

J Am Soc Mass Spectrom. 2020-12-2

[6]
Aza-crown ether locked on polyethyleneimine: solving the contradiction between transfection efficiency and safety during in vivo gene delivery.

Chem Commun (Camb). 2020-5-21

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Folate-conjugated FeO@SiO@gold nanorods@mesoporous SiO hybrid nanomaterial: a theranostic agent for magnetic resonance imaging and photothermal therapy.

J Mater Chem B. 2013-6-21

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Recent Advances in the Use of Focused Ultrasound for Magnetic Resonance Image-Guided Therapeutic Nanoparticle Delivery to the Central Nervous System.

Front Pharmacol. 2019-11-13

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Chem Commun (Camb). 2019-10-23

[10]
Polymeric Carriers for Nucleic Acid Delivery: Current Designs and Future Directions.

Biomacromolecules. 2019-9-20

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