用于肝细胞癌基因递送和磁共振成像的三元杂化纳米复合材料。
Ternary hybrid nanocomposites for gene delivery and magnetic resonance imaging of hepatocellular carcinoma cells.
机构信息
Department of Chemistry and Institute of Creativity, The Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong SAR, P. R. China;
Department of Chemistry and Institute of Creativity, The Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong SAR, P. R. China; ; Department of Imaging and Interventional Radiology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR, P. R. China.
出版信息
Quant Imaging Med Surg. 2013 Dec;3(6):302-7. doi: 10.3978/j.issn.2223-4292.2013.12.05.
Abstract
This paper describes comparative studies in magnetic resonance imaging (MRI) and gene deliveries toward hepatocellular carcinoma (HCC) HepG2 cells with ternary composites that consist of superparamagnetic iron oxide (SPIO) nanoparticles (NPs) (8-10 nm) with deferoxamine coating, circular plasmid DNA (~4 kb) equipped with green fluorescent probe, and branched polyethylenimine (PEI) (25 kDa, PDI 2.5). The packaging of the ternary complexes has been characterized by agarose gel retardation assay. By tuning the PEI/NP ratios and with a fixed DNA amount, different ternary composites have been employed for NP/gene transfection towards HepG2 cells, which have been characterized by in vitro MRI and green fluorescence protein (GFP) fluorescence.
摘要
本文描述了对比研究磁共振成像（MRI）和基因传递对肝癌（HCC）HepG2 细胞的三元复合物，由超顺磁性氧化铁（SPIO）纳米粒子（NP）（8-10nm）与去铁胺涂层、带有绿色荧光探针的环状质粒 DNA（~4kb）和支化聚乙烯亚胺（PEI）（25kDa，PDI2.5）组成。三元复合物的包封已通过琼脂糖凝胶阻滞试验进行了表征。通过调整 PEI/NP 比例，并使用固定的 DNA 量，已将不同的三元复合物用于 NP/基因转染 HepG2 细胞，这已通过体外 MRI 和绿色荧光蛋白（GFP）荧光进行了表征。
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