纳米硒通过下调 ACACA 和 FASN 缓解重铬酸钾诱导的 LMH 细胞脂质代谢紊乱。
Nano-selenium alleviating the lipid metabolism disorder of LMH cells induced by potassium dichromate via down-regulating ACACA and FASN.
机构信息
Veterinary Clinical Medicine Laboratory, College of Veterinary Medicine, Huazhong Agricultural University, Shizishan Street, Wuhan, 430070, People's Republic of China.
出版信息
Environ Sci Pollut Res Int. 2021 Dec;28(48):69426-69435. doi: 10.1007/s11356-021-14775-9. Epub 2021 Jul 23.
Chromium (Cr) VI is a common environmental contaminant highly toxic to livers. To explore the protective effect of nano-selenium (NANO-Se) on broiler liver damage caused by Cr (VI), this experiment was conducted with chicken hepatocellular carcinoma cell line (LMH) as the research object, using potassium dichromate (PDC) and NANO-Se gel for culturing cells. The results indicated that: (1) in the PDC-exposure group, LMH cells being treated with 20 μmol/L PDC for 24 h, IC50 (median inhibition concentration) = 23.427 could significantly reduce cell activity (p < 0.01) which decreased over time. PDC markedly increased the concentration of triglycerides (TG) and low-density lipoprotein cholesterol (LDL-C) in LMH cells (p < 0.01), which increased over time. In addition, PDC could substantially augment the transcription and protein levels of acetyl-CoA carboxylases alpha (ACACA) and fatty acid synthase (FASN) in LMH cells (p < 0.01). (2) Compared with the PDC-exposure group, the addition of 8 μmol/L NANO-Se after 12 h of PDC treatment could significantly increase the cell viability (p < 0.01) but decreased over time; the levels of TG and LDL-C in LMH cells declined markedly (p < 0.01). In addition, the transcription and protein levels of ACACA and FASN in LMH cells were significantly reduced (p < 0.01). (3) The LMH cells were cultured in advance with 8 μmol/L NANO-Se for 12 h and then with PDC for 24 h. The obtained results were similar to the above. There were no obvious differences in TG and LDL-C levels (p > 0.05). However, significant differences were found in the activity of LMH cells and the expression of genes related to lipid metabolism (p < 0.05).All these results suggest that the exposure to PDC promotes the increase of lipid synthesis in LMH cells and causes disorders in the lipid metabolism. Moreover, NANO-Se can partially attenuate the damage caused by PDC through down-regulating of the lipid metabolism-related genes (ACACA and FASN) in LMH cells.
六价铬（Cr VI）是一种常见的环境污染物，对肝脏有剧毒。为了探索纳米硒（NANO-Se）对六价铬（Cr VI）引起的肉鸡肝损伤的保护作用，本实验以鸡肝癌细胞系（LMH）为研究对象，采用重铬酸钾（PDC）和 NANO-Se 凝胶培养细胞。结果表明：（1）在 PDC 暴露组中，用 20 μmol/L PDC 处理 LMH 细胞 24 h，IC50（中值抑制浓度）= 23.427，可显著降低细胞活力（p < 0.01），且随着时间的推移而降低。PDC 显著增加 LMH 细胞中甘油三酯（TG）和低密度脂蛋白胆固醇（LDL-C）的浓度（p < 0.01），且随着时间的推移而增加。此外，PDC 可显著增加 LMH 细胞中乙酰辅酶 A 羧化酶α（ACACA）和脂肪酸合酶（FASN）的转录和蛋白水平（p < 0.01）。（2）与 PDC 暴露组相比，PDC 处理 12 h 后添加 8 μmol/L NANO-Se 可显著提高细胞活力（p < 0.01），但随着时间的推移而降低；LMH 细胞中 TG 和 LDL-C 的水平显著降低（p < 0.01）。此外，LMH 细胞中 ACACA 和 FASN 的转录和蛋白水平显著降低（p < 0.01）。（3）将 LMH 细胞预先用 8 μmol/L NANO-Se 培养 12 h，然后用 PDC 培养 24 h，得到的结果与上述相似。TG 和 LDL-C 水平无明显差异（p > 0.05）。然而，LMH 细胞的活性和与脂质代谢相关的基因表达存在显著差异（p < 0.05）。综上所述，PDC 的暴露促进了 LMH 细胞中脂质合成的增加，并导致脂质代谢紊乱。此外，NANO-Se 可以通过下调 LMH 细胞中与脂质代谢相关的基因（ACACA 和 FASN），部分减轻 PDC 引起的损伤。
Zotero 插件