Veterinary Clinical Medicine Laboratory, College of Veterinary Medicine, Huazhong Agricultural University, Shizishan Street, Wuhan, 430070, People's Republic of China.
Environ Sci Pollut Res Int. 2021 Dec;28(48):69426-69435. doi: 10.1007/s11356-021-14775-9. Epub 2021 Jul 23.
Chromium (Cr) VI is a common environmental contaminant highly toxic to livers. To explore the protective effect of nano-selenium (NANO-Se) on broiler liver damage caused by Cr (VI), this experiment was conducted with chicken hepatocellular carcinoma cell line (LMH) as the research object, using potassium dichromate (PDC) and NANO-Se gel for culturing cells. The results indicated that: (1) in the PDC-exposure group, LMH cells being treated with 20 μmol/L PDC for 24 h, IC50 (median inhibition concentration) = 23.427 could significantly reduce cell activity (p < 0.01) which decreased over time. PDC markedly increased the concentration of triglycerides (TG) and low-density lipoprotein cholesterol (LDL-C) in LMH cells (p < 0.01), which increased over time. In addition, PDC could substantially augment the transcription and protein levels of acetyl-CoA carboxylases alpha (ACACA) and fatty acid synthase (FASN) in LMH cells (p < 0.01). (2) Compared with the PDC-exposure group, the addition of 8 μmol/L NANO-Se after 12 h of PDC treatment could significantly increase the cell viability (p < 0.01) but decreased over time; the levels of TG and LDL-C in LMH cells declined markedly (p < 0.01). In addition, the transcription and protein levels of ACACA and FASN in LMH cells were significantly reduced (p < 0.01). (3) The LMH cells were cultured in advance with 8 μmol/L NANO-Se for 12 h and then with PDC for 24 h. The obtained results were similar to the above. There were no obvious differences in TG and LDL-C levels (p > 0.05). However, significant differences were found in the activity of LMH cells and the expression of genes related to lipid metabolism (p < 0.05).All these results suggest that the exposure to PDC promotes the increase of lipid synthesis in LMH cells and causes disorders in the lipid metabolism. Moreover, NANO-Se can partially attenuate the damage caused by PDC through down-regulating of the lipid metabolism-related genes (ACACA and FASN) in LMH cells.
六价铬（Cr VI）是一种常见的环境污染物，对肝脏有剧毒。为了探索纳米硒（NANO-Se）对六价铬（Cr VI）引起的肉鸡肝损伤的保护作用，本实验以鸡肝癌细胞系（LMH）为研究对象，采用重铬酸钾（PDC）和 NANO-Se 凝胶培养细胞。结果表明：（1）在 PDC 暴露组中，用 20 μmol/L PDC 处理 LMH 细胞 24 h，IC50（中值抑制浓度）= 23.427，可显著降低细胞活力（p < 0.01），且随着时间的推移而降低。PDC 显著增加 LMH 细胞中甘油三酯（TG）和低密度脂蛋白胆固醇（LDL-C）的浓度（p < 0.01），且随着时间的推移而增加。此外，PDC 可显著增加 LMH 细胞中乙酰辅酶 A 羧化酶α（ACACA）和脂肪酸合酶（FASN）的转录和蛋白水平（p < 0.01）。（2）与 PDC 暴露组相比，PDC 处理 12 h 后添加 8 μmol/L NANO-Se 可显著提高细胞活力（p < 0.01），但随着时间的推移而降低；LMH 细胞中 TG 和 LDL-C 的水平显著降低（p < 0.01）。此外，LMH 细胞中 ACACA 和 FASN 的转录和蛋白水平显著降低（p < 0.01）。（3）将 LMH 细胞预先用 8 μmol/L NANO-Se 培养 12 h，然后用 PDC 培养 24 h，得到的结果与上述相似。TG 和 LDL-C 水平无明显差异（p > 0.05）。然而，LMH 细胞的活性和与脂质代谢相关的基因表达存在显著差异（p < 0.05）。综上所述，PDC 的暴露促进了 LMH 细胞中脂质合成的增加，并导致脂质代谢紊乱。此外，NANO-Se 可以通过下调 LMH 细胞中与脂质代谢相关的基因（ACACA 和 FASN），部分减轻 PDC 引起的损伤。
