College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China.
Environ Sci Pollut Res Int. 2019 Jul;26(21):21828-21834. doi: 10.1007/s11356-019-05397-3. Epub 2019 May 27.
The effect of selenium on excessive fatty acid-induced apoptosis and abnormal amino acid metabolism in the liver is well known, because it is an important site in the fatty acid metabolism pathway. However, the protective role of nano-elemental selenium (nano-Se) supplementation against hexavalent chromium (KCrO)-induced abnormal fatty acid metabolism has not been evaluated yet. Therefore, we conducted chicken experiments with different nano-Se supplementation doses to investigate the role of nano-Se against Cr(VI)-induced adverse effects. For this purpose, a total of 120 1-day-old chicks were randomly divided into control group, Cr(VI)-exposed group, protection group, treatment group, prevention group, and nano-Se control group. The results of RT-qPCR showed that the nano-Se supplementation notably downregulated (P < 0.01) the messenger RNA (mRNA) expression levels of fatty acid synthase (FASN), whereas nano-Se supplementation significantly upregulated (P < 0.01) the mRNA expression level of acyl-coenzyme A oxidase 1 (ACOX1) in chicken's liver at day 35 of the experiment. Similar results were further verified by western blot analysis. Moreover, nano-Se supplementation significantly enhanced and reduced the antibody expression levels of ACOX1 and FASN in immunohistochemical analysis, respectively. Thus, finally, it was concluded that nano-Se can alleviate KCrO-induced abnormal fatty acid metabolism in chicken's liver.
硒对脂肪酸诱导的细胞凋亡和肝脏中异常氨基酸代谢的影响是众所周知的，因为它是脂肪酸代谢途径中的一个重要部位。然而，纳米硒（nano-Se）补充对六价铬（KCrO）诱导的异常脂肪酸代谢的保护作用尚未得到评估。因此，我们进行了不同纳米硒补充剂量的鸡实验，以研究纳米硒对 Cr(VI)诱导的不良影响的作用。为此，共选择了 120 只 1 日龄雏鸡，随机分为对照组、Cr(VI)暴露组、保护组、治疗组、预防组和纳米硒对照组。实时荧光定量聚合酶链反应（RT-qPCR）的结果表明，纳米硒补充剂显著下调（P < 0.01）了鸡肝中脂肪酸合成酶（FASN）的信使 RNA（mRNA）表达水平，而纳米硒补充剂在实验第 35 天显著上调（P < 0.01）了酰基辅酶 A 氧化酶 1（ACOX1）的 mRNA 表达水平。western blot 分析进一步验证了类似的结果。此外，免疫组织化学分析表明，纳米硒补充剂显著增强和降低了 ACOX1 和 FASN 的抗体表达水平。因此，最终得出结论，纳米硒可以缓解 KCrO 诱导的鸡肝异常脂肪酸代谢。
