Hygiene Unit, San Martino Policlinico Hospital, IRCCS for Oncology and Neurosciences, Largo Rosanna Benzi 10, 16132, Genoa, Italy.
Department of Health Sciences (DISSAL), University of Genoa, Via Pastore 1, 16132, Genoa, Italy.
Arch Virol. 2021 Oct;166(10):2825-2828. doi: 10.1007/s00705-021-05165-0. Epub 2021 Jul 24.
Extraction-based real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) is currently the "gold standard" in SARS-CoV-2 diagnostics. However, some extraction-free RT-qPCR techniques have recently been developed. In this study, we compared the sensitivity of traditional extraction-based, heated extraction-free, and unheated extraction-free RT-qPCR methods for SARS-CoV-2 detection in nasopharyngeal swabs from symptomatic individuals. The unheated extraction-free method showed perfect agreement with the standard extraction-based RT-qPCR. By contrast, the heat-treated technique was associated with an 8.2% false negativity rate. Unheated extraction-free RT-qPCR for the molecular diagnosis of SARS-CoV-2 is a valuable alternative to the traditional extraction-based methods and may accelerate turnaround times by about two hours.
基于提取的实时逆转录定量聚合酶链反应 (RT-qPCR) 目前是 SARS-CoV-2 诊断的“金标准”。然而,最近已经开发出一些无需提取的 RT-qPCR 技术。在这项研究中,我们比较了传统基于提取、加热无需提取和非加热无需提取 RT-qPCR 方法在检测有症状个体鼻咽拭子中 SARS-CoV-2 的灵敏度。非加热无需提取方法与标准基于提取的 RT-qPCR 具有完美的一致性。相比之下,热处理技术与 8.2% 的假阴性率相关。非加热无需提取 RT-qPCR 是 SARS-CoV-2 分子诊断的一种有价值的替代方法,可能会将周转时间缩短约两个小时。