一株詹氏甲烷球菌来源的 sn-1,3 位特异性脂肪酶用于高效合成长中长型结构三酰基甘油。
A novel sn-1,3 specific lipase from Janibacter sp. as catalysts for the high-yield synthesis of long-medium-long type structured triacylglycerols.
机构信息
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China.
出版信息
Food Chem. 2022 Jan 1;366:130523. doi: 10.1016/j.foodchem.2021.130523. Epub 2021 Jul 5.
Our study offers a novel sn-1,3 specific lipase MAJ1 from marine member Janibacter sp. strain HTCC2649 for preparing long-medium-long (LML) type structured triacylglycerols (TAGs). Firstly, the resin ECR1030 was selected as a suitable support for the immobilization of lipase MAJ1. An efficient synthesis of LML-type structured TAGs by the immobilized lipase MAJ1-catalyzed interesterification of methyl palmitate and tricaprylin was studied in a solvent-free system. The reaction conditions, including substrate molar ratio, temperature and enzyme loading, were optimized. Under the optimum conditions (immobilized lipase MAJ1 of 45 U/g, substrate molar ratio of 4:1, temperature of 35 °C, reaction time of 24 h), the structured TAGs with double long chains (DLCST) were obtained in a yield of 44.3 mol%. Secondly, multi-dimensional mass spectrometry-based shotgun lipidomics (MDMS-SL) was employed to quantify each TAG positional isomer in DLCST. The content of 1,3-dipalmitoyl-2-capryloyl-sn-glycerol in DLCST was 97.6% determined by the MDMS-SL technology.
我们的研究提供了一种新型的海洋分枝杆菌菌株 HTCC2649 来源的 sn-1,3 特异性脂肪酶 MAJ1，可用于制备长-中-长（LML）型结构三酰基甘油(TAGs)。首先，选择树脂 ECR1030 作为脂肪酶 MAJ1 固定化的合适载体。在无溶剂体系中，通过固定化脂肪酶 MAJ1 催化棕榈酸甲酯和三辛酸酯的酯交换反应，研究了 LML 型结构 TAGs 的高效合成。考察了反应条件，包括底物摩尔比、温度和酶用量等对反应的影响，并对反应条件进行了优化。在最佳条件下（固定化脂肪酶 MAJ1 用量为 45 U/g，底物摩尔比为 4:1，温度为 35°C，反应时间为 24 h），以 44.3 mol%的产率得到了双长链结构 TAG（DLCST）。其次，采用基于多维质谱的 shotgun 脂质组学（MDMS-SL）技术对 DLCST 中的每个 TAG 位置异构体进行定量分析。利用 MDMS-SL 技术确定 DLCST 中 1,3-二棕榈酰-2-辛酸-sn-甘油的含量为 97.6%。
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