脂肪酶催化裂解法制备富含二十二碳六烯酸的中长链甘油三酯

Preparation of DHA-Rich Medium- and Long-Chain Triacylglycerols by Lipase-Catalyzed Acidolysis of Microbial Oil from Schizochytrium sp.with Medium-Chain Fatty Acids.

机构信息

State Key Laboratory of Food Science and Technology, National Engineering Research Center for Functional Food, National Engineering Laboratory for Cereal Fermentation Technology, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China.

Joint Laboratory of Functional Food for Healthy Body Fat Loss, Chengdu Tianyi Cuisine Nutritious Food Co., Ltd, 360 Tianhui Road, High-tech Zone, Chengdu, Sichuan, China.

出版信息

Appl Biochem Biotechnol. 2020 Jul;191(3):1294-1314. doi: 10.1007/s12010-020-03261-6. Epub 2020 Feb 25.

DOI:10.1007/s12010-020-03261-6

PMID:32096059

Abstract

DHA-rich medium- and long-chain triacylglycerols (MLCT) were produced by lipase-catalyzed acidolysis of microbial oil from Schizochytrium sp. with medium-chain fatty acids (MCFA). Four commercial lipases, i.e., NS40086, Novozym 435, Lipozyme RM IM, and Lipozyme TL IM were screened based on their activity and fatty acid specificity. The selected conditions for MLCT synthesis were Lipozyme RM IM as catalyst, reaction time 6 h, lipase load 8 wt%, substrate molar ratio (MCFA/microbial oil) 3:1, and temperature 55 °C. Under the selected conditions, the lipase could be reused successively for 17 cycles without significant loss of lipase activity. The obtained product contained 27.53% MCFA, 95.29% at sn-1,3 positions, and 44.70% DHA, 69.77% at sn-2 position. Fifty-nine types of triacylglycerols (TAG) were identified, in which 35 types of TAG contained MCFA, the content accounting for 55.35%. This product enriched with DHA at sn-2 position and MCFA at sn-1,3 positions can improve its digestion and absorption under an infant's digestive system, and thus has potential to be used in infant formula to increase the bioavailability of DHA.

摘要

DHA 丰富的中长链三酰基甘油（MLCT）是通过脂肪酶催化裂解法用中链脂肪酸（MCFA）对裂殖壶菌微生物油进行酸解制备的。根据活性和脂肪酸特异性，筛选了四种商业脂肪酶，即 NS40086、Novozym 435、Lipozyme RM IM 和 Lipozyme TL IM。MLCT 合成的选择条件为 Lipozyme RM IM 为催化剂，反应时间 6 h，脂肪酶用量 8 wt%，底物摩尔比（MCFA/微生物油）为 3:1，温度为 55°C。在选定的条件下，该脂肪酶可以连续重复使用 17 次，而脂肪酶活性没有明显损失。所得产物含有 27.53%的 MCFA、95.29%的 sn-1,3 位、44.70%的 DHA 和 69.77%的 sn-2 位。鉴定出 59 种三酰基甘油（TAG），其中 35 种 TAG 含有 MCFA，含量占 55.35%。这种在 sn-2 位富含 DHA 和在 sn-1,3 位富含 MCFA 的产品可以改善其在婴幼儿消化系统中的消化和吸收，因此有可能在婴幼儿配方奶粉中使用，以提高 DHA 的生物利用度。