石墨、石墨烯和氧化石墨烯对污水污泥和餐厨垃圾厌氧共消化的影响：关注甲烷生成和抗生素抗性基因的归宿。
Effects of graphite, graphene, and graphene oxide on the anaerobic co-digestion of sewage sludge and food waste: Attention to methane production and the fate of antibiotic resistance genes.
机构信息
State Environmental Protection Key Laboratory of Food Chain Pollution Control, School of Ecology and Environment, Beijing Technology and Business University, Beijing 100048, China.
CIECC Overseas Consulting Co., Ltd, Beijing 100048, China.
出版信息
Bioresour Technol. 2021 Nov;339:125585. doi: 10.1016/j.biortech.2021.125585. Epub 2021 Jul 17.
This study explored and compared the influence of graphite, graphene, and graphene oxide (GO) on the performance of anaerobic co-digestion fed with sewage sludge and food waste, the variations of antibiotic resistance genes (ARGs), and the evolution of microbial community. Graphene exhibited the best performance for improving methane production and organic degradation, which increased by 36.09% and 23.07% compared with control group. The experimental results showed that graphene had the greatest influence on the removal efficiency of blaOXA-1, macrolide resistance genes (ermF and ermB), and some tetracycline resistance genes (tetQ and tetX); however, the removal efficiency of sulfonamide resistance genes (sul1 and sul2), intI1, and some tetracycline resistance genes (tetM, tetO, and tetW) were highest when GO was added. Network analysis indicated that the host cells of mefA, ermB, and tetO were different from other ARG host cells; moreover, graphene controlled the horizontal transfer of ARGs between microbial communities.
本研究探讨并比较了石墨、石墨烯和氧化石墨烯（GO）对以污水污泥和食物废物为原料的厌氧共消化性能、抗生素抗性基因（ARGs）的变化以及微生物群落演变的影响。与对照组相比，石墨烯在提高甲烷产量和有机降解方面表现最佳，分别提高了 36.09%和 23.07%。实验结果表明，石墨烯对 blaOXA-1、大环内酯类抗性基因（ermF 和 ermB）和一些四环素类抗性基因（tetQ 和 tetX）的去除效率影响最大；然而，当添加 GO 时，磺胺类抗性基因（sul1 和 sul2）、intI1 和一些四环素类抗性基因（tetM、tetO 和 tetW）的去除效率最高。网络分析表明，mefA、ermB 和 tetO 的宿主细胞与其他 ARG 宿主细胞不同；此外，石墨烯控制了 ARGs 在微生物群落之间的水平转移。
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