Chen Suixia, Wang Xiao, Zheng Shengfeng, Li Hongwen, Qin Shouxu, Liu Jiayi, Jia Wenxian, Shao Mengnan, Tan Yanjun, Liang Hui, Song Weiru, Lu Shaoming, Liu Chengwu, Yang Xiaoli
Scientific Research Center, Guilin Medical University, Guilin, Guangxi 541100, P.R. China.
Guangxi Health Commission Key Laboratory of Disease Proteomics Research, Guilin Medical University, Guilin, Guangxi 541100, P.R. China.
Exp Ther Med. 2021 Sep;22(3):923. doi: 10.3892/etm.2021.10355. Epub 2021 Jun 30.
SPC24 is a crucial component of the mitotic checkpoint machinery in tumorigenesis. High levels of SPC24 have been found in various cancers, including breast cancer, lung cancer, liver cancer, osteosarcoma and thyroid cancer. However, to the best of our knowledge, the impact of SPC24 on prostate cancer (PCa) and other prostate diseases remains unclear. In the present study expression of global SPC24 messenger RNA (mRNA) was assessed in a subset of patients with PCa included in The Cancer Genome Atlas (TCGA) database. Increased levels of SPC24 expression were found in PCa patients >60 years old compared to patients <60 and increased SPC24 expression was also associated with higher levels of prostate specific antigen (P<0.05) and lymph node metastasis (P<0.05). Higher levels of SPC24 expression were associated with negative outcomes in PCa patients (P<0.05). Furthermore, in Chinese patients with prostatitis, benign prostatic hypertrophy (BPH) and PCa, SPC24 was expressed at significantly higher levels than that in adjacent/normal tissues, as assessed by reverse transcription-quantitative polymerase chain reaction, immunohistochemistry and western blotting. High expression of SPC24 was associated with high Gleason stages (IV and V; P<0.05). Further analysis, based on Gene Ontology and pathway functional enrichment analysis, suggested that nuclear division cycle 80 (NDC80), an SPC24 protein interaction partner, and mitotic spindle checkpoint serine/threonine-protein kinase BUB1 (BUB1), a core subunit of the spindle assembly checkpoint, may be associated with SPC24 in PCa development. Finally, using binary logistic regression, algorithms combining the receiver operating characteristic between SPC24 and BUB1 or NDC80 indicated that a combination of these markers may provide better PCa diagnosis ability than other PCa diagnosis markers. Taken together, these findings suggest that SPC24 may be a promising prostate disease biomarker.
SPC24是肿瘤发生过程中有丝分裂检查点机制的关键组成部分。在包括乳腺癌、肺癌、肝癌、骨肉瘤和甲状腺癌在内的多种癌症中均发现SPC24水平较高。然而，据我们所知，SPC24对前列腺癌（PCa）和其他前列腺疾病的影响仍不清楚。在本研究中，对癌症基因组图谱（TCGA）数据库中一部分PCa患者的整体SPC24信使核糖核酸（mRNA）表达进行了评估。与60岁以下患者相比，60岁以上PCa患者的SPC24表达水平升高，且SPC24表达增加还与更高水平的前列腺特异性抗原（P<0.05）和淋巴结转移（P<0.05）相关。SPC24表达水平较高与PCa患者的不良预后相关（P<0.05）。此外，通过逆转录定量聚合酶链反应、免疫组织化学和蛋白质印迹法评估发现，在中国前列腺炎、良性前列腺增生（BPH）和PCa患者中，SPC24的表达水平明显高于相邻/正常组织。SPC24的高表达与高Gleason分级（IV级和V级；P<0.05）相关。基于基因本体论和通路功能富集分析的进一步分析表明，SPC24蛋白相互作用伙伴核分裂周期80（NDC80）以及纺锤体组装检查点的核心亚基有丝分裂纺锤体检查点丝氨酸/苏氨酸蛋白激酶BUB1（BUB1）可能在PCa发展过程中与SPC24相关。最后，使用二元逻辑回归分析，结合SPC24与BUB1或NDC80之间的受试者工作特征曲线的算法表明，这些标志物的组合可能比其他PCa诊断标志物具有更好的PCa诊断能力。综上所述，这些发现表明SPC24可能是一种有前景的前列腺疾病生物标志物。
