人乳头状甲状腺癌和间变性甲状腺癌中Toll样受体、髓样分化因子88及TIR结构域衔接蛋白的免疫组织化学分析

Immunohistochemical Analysis of Toll-Like Receptors, MyD88, and TRIF in Human Papillary Thyroid Carcinoma and Anaplastic Thyroid Carcinoma.

作者信息

Nihon-Yanagi Yasuhiro, Wakayama Megumi, Tochigi Naobumi, Saito Fumi, Ogata Hideaki, Shibuya Kazutoshi

机构信息

Department of Surgical Pathology, Toho University, Omori Hospital, 6-11-1 Omori Nishi, Ota-Ku, Tokyo 143-8541, Japan.

Division of Breast and Endocrine Surgery, Department of Surgery, Toho University, Omori Hospital, 6-11-1 Omori Nishi, Ota-Ku, Tokyo 143-8541, Japan.

出版信息

J Thyroid Res. 2021 Jul 1;2021:4226491. doi: 10.1155/2021/4226491. eCollection 2021.

DOI:10.1155/2021/4226491

PMID:34306609

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8270699/

Abstract

PURPOSE

We hypothesized that innate immune response pathways might be involved in thyroid carcinogenesis. To investigate this hypothesis, we aimed at analyzing the expression of several receptors and molecules in the innate immune system in papillary thyroid carcinoma (PTC) and anaplastic thyroid carcinoma (ATC) tissues.

METHODS

Of the surgically resected specimens, 11 ATC tissues, 25 PTC tissues, and 8 nodular hyperplasia (NH) tissues were selected and examined for the expression of toll-like receptor (TLR) 2, TLR3, TLR4, TLR5, TLR7, TLR9, the myeloid differentiation primary response gene 88 (MyD88), and toll-interleukin-1 receptor domain-containing adaptor inducing INF- (TRIF) by immunohistochemistry (IHC).

RESULTS

Several TLRs were expressed in each tissue. TLR3 was strongly expressed in all tissues. In contrast, TLR4 was not detected in any tissues. While TLR5 was moderately expressed in NH but significantly reduced in PTC and ATC, TLR9 was absent in NH tissue but moderately expressed in both PTC and ATC. On MyD88 expression, no significant difference was found between PTC and ATC. TRIF was significantly upregulated in PTC and ATC compared to NH. Surprisingly, PTC and ATC tissues exhibited similar expression patterns of TLRs, MyD88, and TRIF.

CONCLUSION

These data suggest the involvement of the innate immune system in both PTC and ATC. Specifically, TLR3-mediated TRIF activation was confirmed in PTC and ATC. This provides new insight into thyroid carcinogenesis.

摘要

目的

我们推测先天免疫反应途径可能参与甲状腺癌的发生。为了验证这一推测，我们旨在分析乳头状甲状腺癌（PTC）和未分化甲状腺癌（ATC）组织中先天免疫系统中几种受体和分子的表达情况。

方法

在手术切除的标本中，选取11例ATC组织、25例PTC组织和8例结节性增生（NH）组织，采用免疫组织化学（IHC）检测Toll样受体（TLR）2、TLR3、TLR4、TLR5、TLR7、TLR9、髓样分化初级反应基因88（MyD88）和含Toll白细胞介素-1受体结构域的诱导干扰素β适配器（TRIF）的表达。

结果

各组织中均有几种TLR表达。TLR3在所有组织中均强烈表达。相比之下，在任何组织中均未检测到TLR4。TLR5在NH中中度表达，但在PTC和ATC中显著降低，而TLR9在NH组织中缺失，但在PTC和ATC中均中度表达。关于MyD88表达，PTC和ATC之间未发现显著差异。与NH相比，TRIF在PTC和ATC中显著上调。令人惊讶的是，PTC和ATC组织在TLR、MyD88和TRIF的表达模式上相似。