Rawi Muhamad Hanif, Abdullah Aminah, Ismail Amin, Sarbini Shahrul Razid
Faculty of Agricultural Science and Forestry, Universiti Putra Malaysia Kampus Bintulu Sarawak, Bintulu, Sarawak 97008, Malaysia.
Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi, Selangor 43600, Malaysia.
ACS Omega. 2021 Jul 2;6(28):17782-17797. doi: 10.1021/acsomega.1c00302. eCollection 2021 Jul 20.
Acacia gum (AG) is a branched-polysaccharide gummy exudate that consists of arabinose and galactose. The traditional practice in African-Middle Eastern countries uses this gum as medicine. Traditional use of AG is to treat stomach disease, which can be a potential functional food. In this research, commercially available AG from and was investigated as the prebiotic. The experiment employed a pH-controlled in vitro colon model inoculated with human fecal microbiota to mimic the human colon. Fermentation samples at 0, 6, 12, and 24 h were brought for short-chain fatty acid (SCFA) analysis using high-performance liquid chromatography and bacterial enumeration via fluorescent in situ hybridization. Results showed that AG significantly promotes proliferation similar to fructo-oligosaccharides (FOS) while inhibiting the group, commonly associated with gut dysbiosis. Acetate, propionate, and butyrate showed a similar trend to FOS ( > 0.05). The AG shows potential against gut dysbiosis, as it promotes gut-probiotics, through modulation of microbial population and SCFA production, especially butyrate.
阿拉伯胶（AG）是一种由阿拉伯糖和半乳糖组成的支链多糖胶状渗出物。非洲 - 中东国家的传统做法是将这种胶用作药物。AG的传统用途是治疗胃病，它可能是一种潜在的功能性食品。在这项研究中，对从[具体来源1]和[具体来源2]获得的市售AG作为益生元进行了研究。该实验采用了接种人类粪便微生物群的pH控制体外结肠模型来模拟人类结肠。在0、6、12和24小时采集发酵样品，使用高效液相色谱法进行短链脂肪酸（SCFA）分析，并通过荧光原位杂交进行细菌计数。结果表明，AG显著促进[具体益生菌名称]的增殖，类似于低聚果糖（FOS），同时抑制[具体有害菌名称]组，该组通常与肠道生态失调有关。乙酸盐、丙酸盐和丁酸盐显示出与FOS相似的趋势（P>0.05）。AG通过调节微生物种群和SCFA产生，特别是丁酸盐的产生，对肠道生态失调具有潜在的抵抗作用，因为它能促进肠道益生菌。
