嗜热脂肪芽孢杆菌核糖体的翻译特异性

Translational specificity of Bacillus stearothermophilus ribosomes.

作者信息

Chen C S, Nakamoto T

出版信息

Proc Natl Acad Sci U S A. 1978 Jan;75(1):167-71. doi: 10.1073/pnas.75.1.167.

DOI:10.1073/pnas.75.1.167

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC411206/

Abstract

The translational specificity of Bacillus stearothermophilus ribosomes was studied by determining the effectiveness of various synthetic RNAs as templates at 37 degrees and at higher temperatures. The effectiveness of poly(G,U) was maximal at a G:U ratio of 1:3; it declined with lower G content because of reduced ribosomal affinity for the RNA and, with higher G, because of interference by secondary structure. The effectiveness of poly(A,C,G,U) also declined when secondary structure was increased by increasing (G+C) content. Escherichia coli ribosomes exhibited a similar specificity for poly-(G,U), but had a lower sensitivity to interference by RNA secondary structure. In both bacterial species, sensitivity to secondary structure was determined by the 30S ribosomal subunit.

摘要

通过测定各种合成RNA在37℃及更高温度下作为模板的有效性，研究了嗜热脂肪芽孢杆菌核糖体的翻译特异性。聚（G，U）在G:U比例为1:3时有效性最大；G含量较低时，由于核糖体对RNA的亲和力降低，其有效性下降；G含量较高时，则是由于二级结构的干扰。当通过增加（G+C）含量来增加二级结构时，聚（A，C，G，U）的有效性也会下降。大肠杆菌核糖体对聚（G，U）表现出类似的特异性，但对RNA二级结构干扰的敏感性较低。在这两种细菌中，对二级结构的敏感性由30S核糖体亚基决定。

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