大肠杆菌的突变RNA聚合酶在产生缺陷型ρ因子的菌株中终止转录。
Mutant RNA polymerase of Escherichia coli terminates transcription in strains making defective rho factor.
作者信息
Guarente L P, Beckwith J
出版信息
Proc Natl Acad Sci U S A. 1978 Jan;75(1):294-7. doi: 10.1073/pnas.75.1.294.
Abstract
We have isolated a rifampicin-resistant mutant of Escherichia coli RNA polymerase that restores transcription termination in strains with a defective rho protein. In such strains, the mutant RNA polymerase terminates transcription at normally rho-dependent sites at the end of the trp operon, in bacteriophage lambda, and within the lac operon. In addition, a strain with this mutant RNA polymerase remains viable with an amber mutation in rho, whereas a strain with wild-type RNA polymerase does not. These results suggest that the mutant RNA polymerase can terminate transcription at normally rho-dependent sites in the absence of rho.
摘要
我们分离出了一株大肠杆菌RNA聚合酶的利福平抗性突变体，它能在rho蛋白缺陷的菌株中恢复转录终止。在这类菌株中，突变的RNA聚合酶能在色氨酸操纵子末端、噬菌体λ以及乳糖操纵子内正常依赖rho的位点终止转录。此外，携带这种突变RNA聚合酶的菌株在rho基因发生琥珀突变的情况下仍能存活，而携带野生型RNA聚合酶的菌株则不能。这些结果表明，突变的RNA聚合酶在没有rho的情况下能够在正常依赖rho的位点终止转录。
相似文献
1
Mutant RNA polymerase of Escherichia coli terminates transcription in strains making defective rho factor.
Proc Natl Acad Sci U S A. 1978 Jan;75(1):294-7. doi: 10.1073/pnas.75.1.294.
2
The attenuator of the tryptophan operon in E.coli: rho-mediated release of RNA polymerase from a transcription termination complex in vitro.
Nucleic Acids Res. 1978 Dec;5(12):4613-23.
3
Interaction of RNA polymerase and rho in transcription termination: coupled ATPase.
Proc Natl Acad Sci U S A. 1978 Oct;75(10):4828-32. doi: 10.1073/pnas.75.10.4828.
4
Effect of mutations in the RNA polymerase gene and that of the transcription termination factor rho on expression of the Escherichia coli galactose operon with an IS2 polar insertion.
Mol Gen Genet. 1985;201(2):344-6. doi: 10.1007/BF00425682.
5
The C-terminal domain of the alpha subunit of Escherichia coli RNA polymerase is required for efficient rho-dependent transcription termination.
J Mol Biol. 1998 Dec 18;284(5):1379-90. doi: 10.1006/jmbi.1998.2272.
6
Termination of transcription by Escherichia coli RNA polymerase: influence of secondary structure of RNA transcripts on rho-independent and rho-dependent termination.
Proc Natl Acad Sci U S A. 1979 Apr;76(4):1613-7. doi: 10.1073/pnas.76.4.1613.
7
Escherichia coli RNA polymerase terminates transcription efficiently at rho-independent terminators on single-stranded DNA templates.
Proc Natl Acad Sci U S A. 1997 Dec 9;94(25):13548-53. doi: 10.1073/pnas.94.25.13548.
8
Transcription termination by bacteriophage T7 RNA polymerase at rho-independent terminators.
J Biol Chem. 1990 Mar 5;265(7):3823-30.
9
The mechanism of early transcription termination by Rho026.
J Mol Biol. 1996 Jul 19;260(3):347-58. doi: 10.1006/jmbi.1996.0405.
10
Rho-dependent termination and concomitant NTPase activity requires a specific, intact RNA region.
J Biol Chem. 1984 Feb 25;259(4):2268-73.
引用本文的文献
1
Mastering the control of the Rho transcription factor for biotechnological applications.
Appl Microbiol Biotechnol. 2021 May;105(10):4053-4071. doi: 10.1007/s00253-021-11326-7. Epub 2021 May 8.
2
DNA Breaks-Mediated Fitness Cost Reveals RNase HI as a New Target for Selectively Eliminating Antibiotic-Resistant Bacteria.
Mol Biol Evol. 2021 Jul 29;38(8):3220-3234. doi: 10.1093/molbev/msab093.
3
A Screen for Suppressors Reveals a Key Role for a Connector Region of Termination Factor Rho.
mBio. 2017 May 30;8(3):e00753-17. doi: 10.1128/mBio.00753-17.
4
Transcription termination defective mutants of Rho: role of different functions of Rho in releasing RNA from the elongation complex.
J Mol Biol. 2007 Aug 24;371(4):855-72. doi: 10.1016/j.jmb.2007.06.013. Epub 2007 Jun 9.
5
Rho-dependent transcription termination: more questions than answers.
J Microbiol. 2006 Feb;44(1):11-22.
6
Just toothpicks and logic: how some labs succeed at solving complex problems.
J Bacteriol. 2003 Jan;185(2):387-90. doi: 10.1128/JB.185.2.387-390.2003.
7
Incompatibility of Escherichia coli rho mutants with plasmids is mediated by plasmid-specific transcription.
J Bacteriol. 1997 Sep;179(18):5789-94. doi: 10.1128/jb.179.18.5789-5794.1997.
8
Cloning and expression in Escherichia coli of genetic determinants for production of and immunity to microcin E492 from Klebsiella pneumoniae.
J Bacteriol. 1997 Aug;179(15):4789-94. doi: 10.1128/jb.179.15.4789-4794.1997.
9
RifR mutations in the beginning of the Escherichia coli rpoB gene.
Mol Gen Genet. 1994 Jul 25;244(2):120-6. doi: 10.1007/BF00283512.
10
Streptomycin- and rifampin-resistant mutants of Escherichia coli perturb F exclusion of bacteriophage T7 by affecting synthesis of the F plasmid protein PifA.
J Bacteriol. 1995 Mar;177(6):1589-94. doi: 10.1128/jb.177.6.1589-1594.1995.
本文引用的文献
1
Termination factor for RNA synthesis.
Nature. 1969 Dec 20;224(5225):1168-74. doi: 10.1038/2241168a0.
2
Directed transposition of the arabinose operon: a technique for the isolation of specialized transducing bacteriophages for any Escherichia coli gene.
J Mol Biol. 1969 Aug 28;44(1):117-27. doi: 10.1016/0022-2836(69)90408-2.
3
Inhibition of lacZ gene translation initiation in trp-lac fusion strains.
J Bacteriol. 1974 Mar;117(3):1231-9. doi: 10.1128/jb.117.3.1231-1239.1974.
4
Transcription termination factor rho activity is altered in Escherichia coli with suA gene mutations.
Proc Natl Acad Sci U S A. 1975 May;72(5):1725-8. doi: 10.1073/pnas.72.5.1725.
5
Genetic fusions defining trp and lac operon regulatory elements.
J Mol Biol. 1975 Apr 15;93(3):331-50. doi: 10.1016/0022-2836(75)90281-8.
6
Termination of transcription in bacteriophage lambda. Heterogeneous, 3'-terminal oligo-adenylate additions and the effects of rho factor.
J Biol Chem. 1975 Jun 25;250(12):4755-64.
7
A link between streptomycin and rifampicin mutation.
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2084-7. doi: 10.1073/pnas.72.6.2084.
8
Transcription termination and late control in phage lambda.
Proc Natl Acad Sci U S A. 1975 Sep;72(9):3300-4. doi: 10.1073/pnas.72.9.3300.
9
Specificity of polarity suppression in E. coli: correction of defects in gene N, but not in gene Q, of phage lambda.
Mol Gen Genet. 1976 Oct 18;148(2):175-82. doi: 10.1007/BF00268383.
10
Polarity suppressors increase expression of the wild-type tryptophan operon of Escherichia coli.
J Mol Biol. 1976 May 15;103(2):395-409. doi: 10.1016/0022-2836(76)90319-3.
Zotero 插件