McCarron M, Osborne Y, Story C J, Dempsey J L, Turner D R, Morley A A
Department of Haematology, Flinders Medical Centre, Bedford Park, South Australia.
Mech Ageing Dev. 1987 Dec;41(3):211-8. doi: 10.1016/0047-6374(87)90041-8.
The relationship of lymphocyte proliferative capacity to age was studied using lymphocytes from neonates, from young adults aged 20-30 and from healthy individuals aged 70-90. Mass cultures expanded exponentially and eventually died after a final expansion of 10(19)-10(52). They therefore showed a Hayflick effect, but in contrast to reported findings for other cell types there was no relationship between age and the magnitude of the final expansion. Cytogenetic and molecular studies showed that monoclonality developed in all mass cultures. Study of individual clones also showed exponential growth followed by cessation. The magnitude of the expansion, 10(4)-10(35), was substantially less than that observed for mass cultures, but was related to age. We conclude that lymphocytes have a heterogeneous proliferative potential, that the overall proliferative potential declines with age but that rare cells having extended proliferative potential continue to be present into old age. The development of monoclonality during the course of mass cultures has implications for the interpretation of findings from such cultures since observations drawn from the later stages of culture reflect the properties of rare cells having high proliferative potential and do not necessarily reflect the properties of the overall population.
利用新生儿、20 - 30岁的年轻成年人以及70 - 90岁的健康个体的淋巴细胞，研究了淋巴细胞增殖能力与年龄的关系。大规模培养物呈指数增长，最终在经历10(19)-10(52)的最终扩增后死亡。因此，它们表现出海弗利克效应，但与其他细胞类型的报道结果相反，年龄与最终扩增幅度之间没有关系。细胞遗传学和分子研究表明，所有大规模培养物中均出现了单克隆性。对单个克隆的研究也显示出指数增长，随后停止。扩增幅度为10(4)-10(35)，明显小于大规模培养物中观察到的幅度，但与年龄有关。我们得出结论，淋巴细胞具有异质性增殖潜力，总体增殖潜力随年龄下降，但具有延长增殖潜力的罕见细胞在老年时仍会存在。大规模培养过程中单克隆性的出现对这类培养物结果的解释具有重要意义，因为从培养后期得出的观察结果反映了具有高增殖潜力的罕见细胞的特性，而不一定反映总体群体的特性。
