Student Research Committee, Cellular and Molecular Research Center, School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran.
Cellular and Molecular Research Center, School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran.
Biotech Histochem. 2022 May;97(4):277-283. doi: 10.1080/10520295.2021.1954692. Epub 2021 Jul 27.
We investigated the effects of the NACHT leucine-rich repeat- and PYD-containing proteins (NLRP3) inflammasome, interleukin -18 (IL-18) and interleukin-1 beta (IL-1β) cytokines on the expression of filaggrin-2 (FLG-2) protein in psoriasis patients. Peripheral blood mononuclear cells (PBMC), including T cells, were isolated from psoriasis patients and healthy donors. Ribonucleic acid (RNA) extraction and reverse transcription-polymerase chain reaction (RT-PCR) were performed for all specimens. Immunohistochemical analysis for FLG-2 in normal and psoriatic epidermal tissue also was performed. Western blot was used to separate and identify FLG-2 protein, and immunohistochemical staining was performed to assess FLG-2 expression for psoriasis skin lesions and normal skin. RT-PCR analysis indicated that NLRP3 inflammasome, IL-18 cytokine and IL-1β cytokine expression were increased in psoriatic epidermis compared to normal skin. We found that the expression of FLG-2 was decreased in psoriatic epidermis compared to normal skin. Higher levels of NLRP3 help decrease the FLG-2 level.
我们研究了 NACHT 富含亮氨酸重复和 PYD 结构域蛋白（NLRP3）炎症小体、白细胞介素-18（IL-18）和白细胞介素-1β（IL-1β）细胞因子对银屑病患者丝聚蛋白-2（FLG-2）蛋白表达的影响。从银屑病患者和健康供体中分离外周血单核细胞（PBMC），包括 T 细胞。对所有标本进行 RNA 提取和逆转录-聚合酶链反应（RT-PCR）。还对正常和银屑病表皮组织中的 FLG-2 进行免疫组织化学分析。使用 Western blot 分离和鉴定 FLG-2 蛋白，并进行免疫组织化学染色以评估银屑病皮损和正常皮肤的 FLG-2 表达。RT-PCR 分析表明，与正常皮肤相比，NLRP3 炎症小体、IL-18 细胞因子和 IL-1β 细胞因子在银屑病表皮中表达增加。我们发现与正常皮肤相比，FLG-2 在银屑病表皮中的表达降低。较高水平的 NLRP3 有助于降低 FLG-2 水平。
