Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China; Key Laboratory of Dongting Lake Aquatic Eco-Environmental Control and Restoration of Hunan Province, School of Hydraulic Engineering, Changsha University of Science & Technology, Changsha, 410004, China.
Key Laboratory of Dongting Lake Aquatic Eco-Environmental Control and Restoration of Hunan Province, School of Hydraulic Engineering, Changsha University of Science & Technology, Changsha, 410004, China.
Chemosphere. 2022 Jan;286(Pt 1):131655. doi: 10.1016/j.chemosphere.2021.131655. Epub 2021 Jul 23.
Semi-continuous experiments were carried out in lab-scale continuous stirred tank reactors to evaluate the effects of fermentation temperature (37 ± 1 °C and 55 ± 1 °C) and total solids (TS) contents (3 %, 6 %, and 12 %) on biohydrogen production from the dark fermentations (DF) of rice straw (RS) and the total operation duration was 105 days. The experimental results show that biohydrogen production (0.46-63.60 mL/g VS) from the thermophilic (55 ± 1 °C) DF (TDF) was higher than the mesophilic (37 ± 1 °C) DF (MDF) (0.19-2.13 mL/g VS) at the three TS contents, and achieved the highest of 63.60 ± 2.98 mL/g VS at TS = 6 % in TDF. The pH, NH-N and total volatile fatty acid of fermentation liquids in the TDF were all higher than those in the MDF. The high abundance of lactic acid-producing bacteria resulted in low biohydrogen produced at TS = 3 %. Under the TDF with TS = 6 %, the highest abundance of hydrolytic bacteria (Ruminiclostridium 54.24 %) led to the highest biohydrogen production. The increase of TS content from 6 % to 12 % induced degradation pathway changes from biohydrogen production to methane production. This study demonstrated that butyric acid fermentation was the main pathway to produce biohydrogen from RS in both DFs.
在实验室规模的连续搅拌釜式反应器中进行了半连续实验，以评估发酵温度（37±1°C 和 55±1°C）和总固体（TS）含量（3%、6%和 12%）对水稻秸秆（RS）黑暗发酵生物制氢的影响，总运行时间为 105 天。实验结果表明，在三种 TS 含量下，嗜热（55±1°C）DF（TDF）的生物制氢量（0.46-63.60 mL/g VS）高于中温（37±1°C）DF（MDF）（0.19-2.13 mL/g VS），在 TS=6%时达到最高，为 63.60±2.98 mL/g VS。TDF 中发酵液的 pH 值、NH-N 和总挥发性脂肪酸均高于 MDF。产乳酸细菌的高丰度导致 TS=3%时生物制氢量低。在 TS=6%的 TDF 下，水解菌（瘤胃拟杆菌 54.24%）的丰度最高，导致生物制氢量最高。TS 含量从 6%增加到 12%会导致从生物制氢到甲烷生成的代谢途径变化。本研究表明，在两种 DF 中，丁酸发酵是 RS 产生生物氢的主要途径。
