Swamy Basavalingayya K, Hosamani Ravikumar, Sathasivam Malarvizhi, Chandrashekhar S S, Reddy Uday G, Moger Narayan
Institute of Agricultural Biotechnology (IABT), University of Agricultural Sciences, Dharwad, Karnataka, 580005, India.
Department of Seed Science and Technology, University of Agricultural Sciences, Dharwad, Karnataka, 580005, India.
Sci Rep. 2021 Jul 27;11(1):15303. doi: 10.1038/s41598-021-94771-8.
Hypergravity-an evolutionarily novel environment has been exploited to comprehend the response of living organisms including plants in the context of extra-terrestrial applications. Recently, researchers have shown that hypergravity induces desired phenotypic variability in seedlings. In the present study, we tested the utility of hypergravity as a novel tool in inducing reliable phenotype/s for potential terrestrial crop improvement applications. To investigate, bread wheat seeds (UAS-375 genotype) were subjected to hypergravity treatment (10×g for 12, and 24 h), and evaluated for seedling vigor and plant growth parameters in both laboratory and greenhouse conditions. It was also attempted to elucidate the associated biochemical and hormonal changes at different stages of vegetative growth. Resultant data revealed that hypergravity treatment (10×g for 12 h) significantly enhanced root length, root volume, and root biomass in response to hypergravity. The robust seedling growth phenotype may be attributed to increased alpha-amylase and TDH enzyme activities observed in seeds treated with hypergravity. Elevated total chlorophyll content and Rubisco (55 kDa) protein expression across different stages of vegetative growth in response to hypergravity may impart physiological benefits to wheat growth. Further, hypergravity elicited robust endogenous phytohormones dynamics in root signifying altered phenotype/s. Collectively, this study for the first time describes the utility of hypergravity as a novel tool in inducing reliable root phenotype that could be potentially exploited for improving wheat varieties for better water usage management.
超重力——一种进化上全新的环境已被用于理解包括植物在内的生物体在地球外应用背景下的反应。最近，研究人员表明超重力会在幼苗中诱导出所需的表型变异。在本研究中，我们测试了超重力作为一种新型工具在诱导可靠表型以用于潜在的陆地作物改良应用方面的效用。为了进行研究，对面包小麦种子（UAS - 375基因型）进行超重力处理（10×g，处理12小时和24小时），并在实验室和温室条件下评估幼苗活力和植株生长参数。还试图阐明营养生长不同阶段相关的生化和激素变化。所得数据表明，超重力处理（10×g，处理12小时）显著增加了根长、根体积和根生物量。这种健壮的幼苗生长表型可能归因于在超重力处理的种子中观察到的α -淀粉酶和TDH酶活性增加。在营养生长的不同阶段，超重力导致总叶绿素含量升高和Rubisco（55 kDa）蛋白表达增加，这可能对小麦生长具有生理益处。此外，超重力在根中引发了强大的内源植物激素动态变化，表明表型发生了改变。总体而言，本研究首次描述了超重力作为一种新型工具在诱导可靠的根表型方面的效用，这种表型有可能被用于改良小麦品种以实现更好的水分利用管理。
