Chen Wan-Hao, Han Yan-Feng, Liang Jian-Dong, Liang Zong-Qi
Basic Medical School, Guizhou University of Traditional Chinese Medicine, Guiyang, 550025, Guizhou, People's Republic of China.
Department of Ecology, Institute of Fungus Resources, College of Life Sciences, Guizhou University, Guiyang, 550025, Guizhou, People's Republic of China.
Sci Rep. 2021 Jul 27;11(1):15300. doi: 10.1038/s41598-021-94893-z.
Simplicillium species are commonly found from soil, seawater, rock surface, decayed wood, air and as symbiotic, endophytic, entomopathogenic and mycoparasitic fungi. Minority insect-associated species was reported. Simplicillium coccinellidae, S. hymenopterorum, S. neolepidopterorum and S. scarabaeoidea were introduced as the newly insect-associated species. The phylogenetic analyses of two combined datasets (LSU + RPB1 + TEF and SSU + ITS + LSU) revealed that S. coccinellidae and S. hymenopterorum were both nested in an independent clade. S. neolepidopterorum and S. scarabaeoidea have a close relationship with S. formicidae and S. lepidopterorum, respectively. S. neolepidopterorum can be easily distinguished from S. formicidae by ellipsoidal to cylindrical, solitary conidia which occasionally gather in short imbricate chains. S. scarabaeoidea could be easily distinguished from S. lepodopterorum by having longer phialides and larger conidia. Based on the morphological and phylogenetic conclusion, we determine the four newly generated isolates as new species of Simplicillium and a new combination is proposed in the genus Leptobacillium.
简梗孢属物种常见于土壤、海水、岩石表面、朽木、空气中，作为共生菌、内生菌、昆虫病原菌和真菌寄生菌存在。少数与昆虫相关的物种被报道。球孢简梗孢、膜翅目简梗孢、新鳞翅目简梗孢和金龟子简梗孢被作为新的与昆虫相关的物种引入。对两个合并数据集（LSU + RPB1 + TEF和SSU + ITS + LSU）的系统发育分析表明，球孢简梗孢和膜翅目简梗孢都嵌套在一个独立的分支中。新鳞翅目简梗孢和金龟子简梗孢分别与蚁简梗孢和鳞翅目简梗孢关系密切。新鳞翅目简梗孢可以通过椭圆形至圆柱形、单个的分生孢子（偶尔聚集成短覆瓦状链）与蚁简梗孢轻松区分。金龟子简梗孢可以通过具有更长的瓶梗和更大的分生孢子与鳞翅目简梗孢轻松区分。基于形态学和系统发育结论，我们将四个新产生的分离株确定为简梗孢属新物种，并在细芽孢杆菌属中提出一个新组合。
