Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia.
Department of Periodontology, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia.
F1000Res. 2021 May 24;10:414. doi: 10.12688/f1000research.52624.2. eCollection 2021.
The Epstein-Barr virus (EBV) is gaining interest as a possible agent in the etiology of periodontitis. Previous studies have shown controversy on whether EBV DNA in the subgingival periodontal pockets is associated with periodontitis. The present study aimed to seek the potential relationship between EBV and periodontitis. Samples were taken from gingival crevicular fluid using sterile paper points, and data on sociodemographics, oral health, and periodontal health were recorded. This case-control study of 118 participants included 59 subjects with severe periodontitis and 59 control subjects with mild periodontitis. Quantitative real-time PCR was used to determined EBV load. EBV DNA was detected in 37.3% of the case samples and 18.6% of the control samples. There was no significant difference in a load of EBV DNA between severe and mild periodontitis (p>0.05). The observed load of EBV DNA was up to 4.55x10 copies/mL. The detected EBV DNA was significantly associated with the plaque index and the oral hygiene index (p<0.05). Although no significant association was found, EBV may play a role in periodontitis. The real-time PCR methods can be used to monitor the EBV load in gingival crevicular fluid.
EB 病毒（EBV）作为牙周炎病因的可能病原体引起了人们的关注。先前的研究表明，龈下牙周袋中的 EBV DNA 是否与牙周炎有关存在争议。本研究旨在探讨 EBV 与牙周炎之间的潜在关系。
使用无菌纸尖从牙龈沟液中采集样本，并记录社会人口统计学、口腔健康和牙周健康数据。这项针对 118 名参与者的病例对照研究包括 59 名重度牙周炎患者和 59 名轻度牙周炎对照者。采用实时定量 PCR 检测 EBV 载量。在 37.3%的病例样本和 18.6%的对照样本中检测到 EBV DNA。重度和轻度牙周炎之间 EBV DNA 的载量没有显著差异（p>0.05）。观察到的 EBV DNA 载量高达 4.55x10 拷贝/mL。检测到的 EBV DNA 与菌斑指数和口腔卫生指数显著相关（p<0.05）。
尽管没有发现显著相关性，但 EBV 可能在牙周炎中起作用。实时 PCR 方法可用于监测龈沟液中的 EBV 载量。
