Konstantinidis Antonis, Sakellari Dimitra, Papa Anna, Antoniadis Antonis
Department of Periodontology, School of Dentistry, Aristotle University of Thessaloniki, Greece.
J Periodontal Res. 2005 Aug;40(4):294-8. doi: 10.1111/j.1600-0765.2005.00796.x.
Although herpes viruses have been implicated in the pathogenesis of chronic and aggressive periodontitis, few data in the literature refer to quantification of these viruses in periodontal sites, especially in relation to serological findings.
The aim of the present study was to compare Epstein--Barr virus (EBV) DNA load in subgingival specimens from chronic periodontitis patients and in periodontally healthy subjects, in relation to serologic testing of IgM and IgG antibodies to EBV.
A total of 22 chronic periodontitis patients and 13 controls participated in the present study. Seventy-nine subgingival specimens (one pooled, one from a deep and one from a shallow site), sampled with paper points, were analysed with real-time polymerase chain reaction for EBV. Subjects were also examined for anti-EBV IgG and IgM levels in serum, using an enzyme-linked immunosorbent assay.
One subject was seronegative for EBV. Three subjects (one patient and two controls) displayed anti-EBV IgM. Their data were excluded from further analysis. All three displayed EBV in their subgingival samples. Nine out of the remaining 20 chronic periodontitis patients and 10 out of 11 controls did not display EBV subgingivally. A statistically significant difference in viral load was observed between pooled and shallow-pocket samples from periodontitis patients but not between samples from deep and shallow pockets (Kruskall--Wallis anova, Dunn's multiple comparisons test).
Data from the present study do not strongly support the pathogenetic significance of EBV in chronic periodontitis lesions. The data do, however, suggest that parallel serological assessments provide a useful insight into the association of viruses with periodontal disease.
尽管疱疹病毒与慢性侵袭性牙周炎的发病机制有关，但文献中很少有关于这些病毒在牙周部位定量的资料，特别是与血清学结果相关的资料。
本研究的目的是比较慢性牙周炎患者龈下标本和牙周健康受试者中爱泼斯坦-巴尔病毒（EBV）的DNA载量，并与EBV的IgM和IgG抗体的血清学检测结果相关联。
共有22名慢性牙周炎患者和13名对照参与了本研究。用纸条采集79个龈下标本（一个混合标本、一个深袋标本和一个浅袋标本），采用实时聚合酶链反应分析EBV。还使用酶联免疫吸附测定法检测受试者血清中的抗EBV IgG和IgM水平。
一名受试者EBV血清学阴性。三名受试者（一名患者和两名对照）显示抗EBV IgM。他们的数据被排除在进一步分析之外。这三名受试者的龈下样本中均检测到EBV。其余20名慢性牙周炎患者中有9名以及11名对照中有10名龈下未检测到EBV。牙周炎患者混合样本和浅袋样本之间的病毒载量存在统计学显著差异，但深袋和浅袋样本之间无差异（Kruskal-Wallis方差分析，Dunn多重比较检验）。
本研究数据并不强烈支持EBV在慢性牙周炎病变中的致病意义。然而，这些数据确实表明，并行的血清学评估有助于深入了解病毒与牙周疾病的关联。
