Palmisano A, D'Auria S, Sannia G, Marino G, Tocco G
Dipartimento di Chimica Organica e Biologica, Universita' di Napoli, Italy.
Neuropeptides. 1987 Nov-Dec;10(4):321-7. doi: 10.1016/s0143-4179(87)90074-6.
Preproenkephalin mRNA was analyzed in Neuroblastoma X Glioma, NG 108-15, cells as well as in respective parental cell lines, i.e. mouse Neuroblastoma N18 and rat Glioma C6, using a rat preproenkephalin cDNA as a probe. NG synthesize efficiently a preproenkephalin mRNA, similar in size to that of normal tissues. Of the two parental cell lines, Neuroblastoma seems to synthesize it as well as NG; conversely Glioma cell lines under the same conditions do not appear to synthesize preproenkephalin mRNA with the same efficiency as NG and N18.
使用大鼠前脑啡肽原cDNA作为探针，对神经母细胞瘤X胶质瘤NG 108 - 15细胞以及各自的亲本细胞系，即小鼠神经母细胞瘤N18和大鼠胶质瘤C6中的前脑啡肽原mRNA进行了分析。NG能高效合成一种前脑啡肽原mRNA，其大小与正常组织中的相似。在这两种亲本细胞系中，神经母细胞瘤似乎能像NG一样合成该物质；相反，在相同条件下，胶质瘤细胞系合成前脑啡肽原mRNA的效率似乎不如NG和N18。
