School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea.
Ildong Bioscience, Pyeongtaek 17957, Republic of Korea.
J Microbiol Biotechnol. 2021 Oct 28;31(10):1366-1372. doi: 10.4014/jmb.2106.06068.
Bacterial nanocellulose (BNC) is a biocompatible material with a lot of potential. To make BNC commercially feasible, improvements in its production and surface qualities must be made. Here, we investigated the in situ fermentation and generation of BNC by addition of different cellulosic substrates such as Avicel and carboxymethylcellulose (CMC) and using sp. SFCB22-18. The addition of cellulosic substrates improved BNC production by a maximum of about 5 times and slightly modified its structural properties. The morphological and structural properties of BNC were investigated by using Fourier transform-infrared spectroscopy (FT-IR), scanning electron microscopy and X-ray diffraction. Furthermore, a type-A cellulose-binding protein derived from , CBD3, was used in a novel biological analytic approach to measure the surface crystallinity of the BNC. Because Avicel and CMC may adhere to microfibrils during BNC synthesis or crystallization, cellulose-binding protein could be a useful tool for identifying the crystalline properties of BNC with high sensitivity.
细菌纳米纤维素（BNC）是一种具有很大潜力的生物相容性材料。为了使 BNC 在商业上可行，必须改进其生产和表面质量。在这里，我们研究了通过添加不同的纤维素底物（如微晶纤维素和羧甲基纤维素（CMC））并用 sp. SFCB22-18 原位发酵和生成 BNC。纤维素底物的添加最多可将 BNC 的产量提高约 5 倍，并略微改变其结构特性。通过使用傅里叶变换红外光谱（FT-IR）、扫描电子显微镜和 X 射线衍射来研究 BNC 的形态和结构特性。此外，一种源自 的 A 型纤维素结合蛋白，CBD3，被用于一种新的生物分析方法来测量 BNC 的表面结晶度。由于在 BNC 合成或结晶过程中，微晶纤维素和 CMC 可能会附着在微纤维上，因此纤维素结合蛋白可能是一种非常有用的工具，可以高灵敏度地识别 BNC 的结晶特性。
