Khaskiye A, Gardahaut M F, Fournier Le Ray C, Rouaud T, Renaud D, Le Douarin G H
Groupe de Physiologie Cellulaire, Centre de Recherche de Biologie et Physico-Chimie cellulaires, Faculté des Sciences, Nantes, France.
Pflugers Arch. 1987 Nov;410(4-5):433-40. doi: 10.1007/BF00586522.
The effects of denervation and direct stimulation in fast and slow latissimus dorsii muscles were investigated in chicken. In slow ALD muscle, denervation resulted in an incompleteness of the relaxation, a decrease in MDH and CPK activities and an increase in fast myosin light chains (MLC) accumulation. Direct stimulation at either fast or slow rhythm prevented the effects of denervation on relaxation and CPK activity but was ineffective on MDH activity and fast MLC accumulation. Moreover, direct stimulation of denervated ALD caused rhythm-dependent change in tetanic contraction. In fast PLD muscle, the main changes in muscle properties following denervation were a slowing down of the time course of the twitch and an incompleteness of the relaxation, a decrease in LDH and CPK activities and in LC3F accumulation. Stimulation at a high frequency partly prevented the effects of denervation and resulted in a large accumulation of LC3F, while a low frequency stimulation did not restore the twitch time to peak, increased MDH activity and induced synthesis of slow MLC. This study emphasizes the role of muscle activity and its pattern in some properties of slow and fast chicken muscles following denervation.
在鸡身上研究了去神经支配和直接刺激对快慢不同的背阔肌的影响。在慢速的前锯肌（ALD）中，去神经支配导致松弛不完全、苹果酸脱氢酶（MDH）和肌酸磷酸激酶（CPK）活性降低以及快肌球蛋白轻链（MLC）积累增加。以快或慢节律进行直接刺激可防止去神经支配对松弛和CPK活性的影响，但对MDH活性和快MLC积累无效。此外，对去神经支配的ALD进行直接刺激会导致强直收缩出现节律依赖性变化。在快速的胸外侧后锯肌（PLD）中，去神经支配后肌肉特性的主要变化是抽搐时间进程减慢和松弛不完全、乳酸脱氢酶（LDH）和CPK活性降低以及LC3F积累减少。高频刺激部分地防止了去神经支配的影响，并导致LC3F大量积累，而低频刺激未能使抽搐时间恢复到峰值，增加了MDH活性并诱导了慢MLC的合成。这项研究强调了肌肉活动及其模式在去神经支配后快慢不同的鸡肌肉某些特性中的作用。
