Wang Shuya, Fang Hua, Xie Jianming, Wu Yue, Tang Zhongqi, Liu Zeci, Lv Jian, Yu Jihua
College of Horticulture, Gansu Agricultural University, Lanzhou, China.
Gansu Provincial Key Laboratory of Arid Land Crop Science, Gansu Agricultural University, Lanzhou, China.
Front Plant Sci. 2021 Jul 12;12:709313. doi: 10.3389/fpls.2021.709313. eCollection 2021.
Normal development of plants is inhibited by inadequate light in winter in greenhouses in Northwest China. Growth lamps, using light-emitting diodes (LEDs) with red blue light (7R2B), were used to supplement daylight for 1, 2, and 3 h. Seedling growth, photosynthesis, and photosynthetic product; the Calvin cycle key and sugar metabolism-related enzymes and their encoding genes; and the light signal sensing regulation of key gene expression were studied in greenhouse cucumbers under three treatments to determine the best supplemental light durations to enhance cucumber cultivation in greenhouses in winter. Treatment with LED red and blue light for 3 h significantly promoted the growth and development of cucumbers, root growth, and dry matter accumulation. It improved the photosynthetic rate, photosynthetic pigment content, and light energy utilization efficiency in cucumbers. Supplementation with red and blue LED light for 3 h upregulated the expression levels of key genes encoding the Calvin cycle and enzymes related to sugar metabolism in cucumber leaves, which promoted the synthesis and accumulation of photosynthates. The expression levels of phytochrome B, cryptochrome 1, and hypocotyl 5 in the cucumber leaves were also significantly upregulated after 3 h of light supplementation. Combined LED red and blue light for 3 h should be used to supplement natural light to enhance the cucumber cultivation in greenhouses in winter.
中国西北地区温室中，冬季光照不足会抑制植物的正常发育。采用发出红蓝光（7R2B）的发光二极管（LED）生长灯，分别补充1、2和3小时的日光。研究了三种处理下温室黄瓜的幼苗生长、光合作用和光合产物；卡尔文循环关键酶和糖代谢相关酶及其编码基因；以及关键基因表达的光信号感知调控，以确定冬季温室黄瓜栽培中最佳的补光时长。用LED红蓝光处理3小时显著促进了黄瓜的生长发育、根系生长和干物质积累。它提高了黄瓜的光合速率、光合色素含量和光能利用效率。补充红蓝光3小时上调了黄瓜叶片中卡尔文循环关键酶和糖代谢相关酶编码基因的表达水平，促进了光合产物的合成和积累。补光3小时后，黄瓜叶片中光敏色素B、隐花色素1和下胚轴5的表达水平也显著上调。应使用组合LED红蓝光3小时来补充自然光，以促进冬季温室黄瓜的栽培。
