维生素 D 受体单核苷酸多态性与犬草酸钙尿石症的关系。
The association between single nucleotide polymorphism in vitamin D receptor and calcium oxalate urolithiasis in dogs.
机构信息
Department of Physiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
STAR unit of Renal Biochemistry and Stone Disease, Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
出版信息
J Vet Intern Med. 2021 Sep;35(5):2263-2270. doi: 10.1111/jvim.16225. Epub 2021 Jul 28.
BACKGROUND
Polymorphisms of the vitamin D receptor (VDR) are associated with calcium oxalate (CaOx) nephrolithiasis in humans.
OBJECTIVES
To investigate the association between VDR polymorphisms and susceptibility to CaOx urolithiasis in dogs.
ANIMALS
Thirty-five dogs with CaOx urolithiasis were compared with 40 stone-free dogs.
METHODS
This was a case-control study. Two VDR gene polymorphisms (rs851998024 and rs852900542) were detected by specific TaqMan real-time polymerase chain reaction assay, and their relationship with serum 1,25-dihydroxyvitamin D, serum and urinary electrolyte concentrations was evaluated.
RESULTS
The distribution of the rs852900542 polymorphism was significantly different between the case and the control dogs (x = 6.369, P = .04). Dogs with a CC or CT genotype had an increased risk of CaOx stones than those with the TT genotype (odds ratio = 3.82, 95% confidence interval 1.04-13.98). The CaOx dogs with the TT genotype had a significantly lower urinary calcium-to-creatinine ratio than the CT+CC genotypes. 1,25-(OH)2D concentrations did not differ between the cases and the controls (308.7 ± 217.4 vs 286.7 ± 185.1 pg/mL, P = .45).
CONCLUSIONS AND CLINICAL IMPORTANCE
This finding suggests that vitamin D metabolism might play a role in CaOx stone formation in dogs.
背景
维生素 D 受体 (VDR) 的多态性与人类草酸钙 (CaOx) 肾结石有关。
目的
研究 VDR 多态性与犬 CaOx 尿石症易感性的关系。
动物
35 只患有 CaOx 尿石症的犬与 40 只无结石犬进行比较。
方法
这是一项病例对照研究。通过特异性 TaqMan 实时聚合酶链反应检测 2 个 VDR 基因多态性 (rs851998024 和 rs852900542)，并评估其与血清 1,25-二羟维生素 D、血清和尿电解质浓度的关系。
结果
病例组和对照组犬 rs852900542 多态性的分布差异有统计学意义 (x ² = 6.369, P = 0.04)。CC 或 CT 基因型的犬发生 CaOx 结石的风险高于 TT 基因型 (比值比 3.82，95%置信区间 1.04-13.98)。TT 基因型的 CaOx 犬的尿钙/肌酐比值明显低于 CT+CC 基因型。病例组和对照组的 1,25-(OH)2D 浓度无差异 (308.7 ± 217.4 比 286.7 ± 185.1 pg/mL，P = 0.45)。
结论和临床意义
这一发现表明，维生素 D 代谢可能在犬 CaOx 结石形成中起作用。
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