Luskin Austin C, Lulich Jody P, Gresch Sarah C, Furrow Eva
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, United States.
Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, United States.
Res Vet Sci. 2019 Apr;123:129-134. doi: 10.1016/j.rvsc.2019.01.001. Epub 2019 Jan 4.
People with calcium oxalate (CaOx) urolithiasis and idiopathic hypercalciuria (IH) often have evidence of increased bone resorption, but bone turnover has not previously been investigated in dogs with these conditions. The aim of this study was to determine whether a marker of bone resorption, β-crosslaps, differs between dogs with CaOx urolithiasis and IH compared to controls. This retrospective, cross-sectional study used a canine specific ELISA to measure β-crosslaps concentrations in stored frozen serum samples from 20 dogs with CaOx urolithiasis and IH and 20 breed-, sex-, and age-matched stone-free controls (18 Miniature Schnauzers, 14 Bichons Frise, and 8 Shih Tzus). Dogs with CaOx urolithiasis and IH had lower β-crosslaps concentrations relative to controls (P = .0043), and β-crosslaps had a moderate negative correlation with urinary calcium-to-creatinine ratios (r = -0.44, P = .0044). Miniature Schnauzers had lower β-crosslaps concentrations than the other two breeds (P = .0035). The ELISA had acceptable intra-assay precision, but concentrations decreased when samples were repeatedly assayed over time. Assay recovery rates were also below acceptance criteria. In conclusion, Miniature Schnauzers, Bichons Frise, and Shih Tzus with CaOx urolithiasis and IH have evidence of decreased bone resorption compared to stone-free controls. This suggests that other causes of IH, such as intestinal hyperabsorption of calcium, underlie risk for CaOx urolithiasis in these breeds. Results should be confirmed in larger populations and with other β-crosslaps assays and additional biomarkers of bone turnover. The stability of canine serum β-crosslaps after freeze-thaw cycles and storage at various temperatures requires investigation.
患有草酸钙（CaOx）尿石症和特发性高钙尿症（IH）的人通常有骨吸收增加的证据，但此前尚未对患有这些病症的犬进行骨转换研究。本研究的目的是确定骨吸收标志物β-交联C端肽（β-crosslaps）在患有CaOx尿石症和IH的犬与对照组之间是否存在差异。这项回顾性横断面研究使用犬特异性酶联免疫吸附测定（ELISA）来测量来自20只患有CaOx尿石症和IH的犬以及20只品种、性别和年龄匹配的无结石对照犬（18只迷你雪纳瑞犬、14只卷毛比雄犬和8只西施犬）的储存冷冻血清样本中的β-crosslaps浓度。患有CaOx尿石症和IH的犬相对于对照组的β-crosslaps浓度较低（P = 0.0043），并且β-crosslaps与尿钙肌酐比值呈中度负相关（r = -0.44，P = 0.0044）。迷你雪纳瑞犬的β-crosslaps浓度低于其他两个品种（P = 0.0035）。ELISA具有可接受的批内精密度，但随着时间的推移对样本进行重复测定时浓度会降低。测定回收率也低于验收标准。总之，与无结石对照犬相比，患有CaOx尿石症和IH的迷你雪纳瑞犬、卷毛比雄犬和西施犬有骨吸收减少的证据。这表明在这些品种中，IH的其他原因，如肠道对钙的过度吸收，是CaOx尿石症风险的基础。结果应在更大的群体中以及使用其他β-crosslaps测定方法和额外的骨转换生物标志物进行确认。犬血清β-crosslaps在冻融循环和不同温度下储存后的稳定性需要进行研究。
