Authier B, Albrand J P, Decorps M, Reutenauer H, Rossi A
D.R.F./Service de Physique/R.M.B.M., Centre d'Etudes Nucléaires de Grenoble, France.
Physiol Chem Phys Med NMR. 1987;19(2):83-93.
This study uses 31P NMR as a tool for the study of the capacity of recovery of the rat skeletal muscle after an exercise performed during an acute state of ischaemia. The leg muscle of a rat submitted to a 20 minute exercise period one hour after irreversible femoral artery ligation, manifested a dramatic (75%) decrease in phosphocreatine (PC) content, a less pronounced (30%) decrease in ATP, an accumulation of inorganic phosphate (Pi) and an increase in the phosphomonoester (PME) resonances, in addition to acidosis to pH 6.4. An investigation over a 40 minute post-exercise period using 31P NMR and biochemical analysis led to the following observations: 1. The PC and Pi contents of the muscle experienced no further significant changes, remaining at the level reached by the end of the exercise. 2. The ATP content similarly remained at the level reached at the end of this period, the adenylate charge being 0.91 (controls 0.93). 3. The IMP accumulated during ischaemic exercise remained at its high level. It seems likely that this compound contributes in a large part to the resonances in the PME region of the spectra. 4. Intracellular acidosis persisted despite a decrease in lactate content. The most important finding from this study is that the situation created by ischaemic exercise--as revealed by the NMR spectra--is characterized by a blocking of the main biochemical processes (phosphorylations, purine nucleotide cycle, pH regulation). Such a condition, which does not seem to entail lethal cell injury, could thus be used as a basis for the study via 31P NMR of the therapeutic effect of various treatments.
本研究使用³¹P核磁共振（NMR）作为一种工具，来研究大鼠骨骼肌在急性缺血状态下运动后恢复的能力。在股动脉不可逆结扎一小时后，对大鼠腿部肌肉进行20分钟的运动，结果显示磷酸肌酸（PC）含量急剧下降（75%），ATP下降幅度较小（30%），无机磷酸盐（Pi）积累，磷酸单酯（PME）共振增加，此外pH值降至6.4，出现酸中毒。在运动后40分钟内，使用³¹P NMR和生化分析进行的一项研究得出了以下观察结果：1. 肌肉中的PC和Pi含量没有进一步显著变化，保持在运动结束时达到的水平。2. ATP含量同样保持在这一时期结束时达到的水平，腺苷酸电荷为0.91（对照组为0.93）。3. 缺血运动期间积累的肌苷酸（IMP）保持在高水平。这种化合物似乎在很大程度上导致了光谱中PME区域的共振。4. 尽管乳酸含量下降，但细胞内酸中毒仍然存在。这项研究最重要的发现是，缺血运动所造成的情况——如NMR光谱所示——其特征是主要生化过程（磷酸化、嘌呤核苷酸循环、pH调节）受阻。这样一种情况似乎不会导致致命的细胞损伤，因此可以作为通过³¹P NMR研究各种治疗方法治疗效果的基础。
