Microbiology and Immunology Laboratory, Federal University of Bahia, Anísio Teixeira Campus, Vitória da Conquista, Brazil; Santa Cruz State University, Ilhéus, Brazil.
Microbiology and Immunology Laboratory, Federal University of Bahia, Anísio Teixeira Campus, Vitória da Conquista, Brazil.
Fertil Steril. 2021 Oct;116(4):1030-1039. doi: 10.1016/j.fertnstert.2021.05.108. Epub 2021 Jul 27.
To study the inflammatory profile and genes involved in the response to bacterial infections in women who developed spontaneous abortion in the presence of Ureaplasma parvum.
Cross-sectional study.
A maternal and child referral center.
PATIENT(S): Eighty-nine women with spontaneous abortion and 20 women with normal vaginal delivery (control group) were studied.
INTERVENTION(S): Samples of biopsied placental tissue were collected for Mollicutes detection.
MAIN OUTCOME MEASURE(S): The samples were subjected to histologic analysis, immunohistochemical evaluation for macrophages and lymphocytes, cytokine quantification, and quantitative polymerase chain reaction array to evaluate the expression of 84 genes related to the innate and adaptive immune responses.
RESULT(S): The presence of U. parvum in the abortion group was positively associated with the influx of polymorphonuclear cells in the placental tissue and increased concentrations of interleukin-6 and interleukin-12p70. U. parvum caused downregulation of genes involved in the immune response, such as attraction of immune cells, activation of an inflammatory response, T-helper cell 17 response activation, and activation of the complement system at the beginning and end of pregnancy.
The direct action of U. parvum on placental tissue altered the gestational tolerogenic state, reducing the immune response against pathogens and activating the extrinsic apoptotic pathway, causing spontaneous abortion.
研究在解脲脲原体（Ureaplasma parvum）存在的情况下发生自然流产的女性的炎症特征和对细菌感染的反应相关基因。
横断面研究。
一家母婴转诊中心。
89 名自然流产患者和 20 名正常阴道分娩的妇女（对照组）。
采集活检胎盘组织样本以检测摩拉克斯体。
对样本进行组织学分析、巨噬细胞和淋巴细胞的免疫组织化学评估、细胞因子定量检测以及与先天和适应性免疫反应相关的 84 个基因的定量聚合酶链反应（PCR）阵列评估。
流产组中解脲脲原体的存在与胎盘组织中多形核细胞的涌入以及白细胞介素-6 和白细胞介素-12p70 浓度的增加呈正相关。解脲脲原体导致与免疫反应相关的基因下调，如免疫细胞的趋化、炎症反应的激活、辅助性 T 细胞 17 反应的激活以及妊娠开始和结束时补体系统的激活。
解脲脲原体对胎盘组织的直接作用改变了妊娠的免疫耐受状态，降低了对病原体的免疫反应，并激活了细胞外凋亡途径，导致自然流产。
