流体中心脏瓣膜组织的显微CT成像
MicroCT Imaging of Heart Valve Tissue in Fluid.
作者信息
Stephens S E, Bean M, Surber H, Ingels N B, Jensen H K, Liachenko S, Wenk J F, Jensen M O
机构信息
Department of Biomedical Engineering, University of Arkansas, Fayetteville, Arkansas, USA.
Departments of Radiology and Surgery, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
出版信息
Exp Mech. 2021 Jan;61(1):253-261. doi: 10.1007/s11340-020-00667-5. Epub 2020 Oct 27.
BACKGROUND
Heart valve computational models require high quality geometric input data, commonly obtained using micro-computed tomography. Whether in the open or closed configuration, most studies utilize dry valves, which poses significant challenges including gravitational and surface tension effects along with desiccation induced mechanical changes.
OBJECTIVE
These challenges are overcome by scanning in a stress-free configuration in fluid. Utilizing fluid backgrounds however reduces overall contrast due to the similar density of fluid and tissue.
METHODS
The work presented here demonstrates imaging of the mitral valve by utilizing an iodine-based staining solution to improve the contrast of valve tissue against a fluid background and investigates the role of stain time and concentration.
RESULTS
It is determined that an Olea europaea oil bath with a relatively high concentration, short stain time approach produces high quality imagery suitable for creating accurate 3D renderings.
CONCLUSIONS
Micro-CT scanning of heart valves in fluid is shown to be feasible using iodine staining techniques.
背景
心脏瓣膜计算模型需要高质量的几何输入数据，通常使用微型计算机断层扫描来获取。无论是处于开放还是闭合状态，大多数研究都使用干燥瓣膜，这带来了重大挑战，包括重力和表面张力效应以及干燥引起的机械变化。
目的
通过在流体中以无应力状态进行扫描来克服这些挑战。然而，由于流体和组织密度相似，利用流体背景会降低整体对比度。
方法
本文介绍的工作展示了通过使用基于碘的染色溶液来改善瓣膜组织在流体背景下的对比度从而对二尖瓣进行成像，并研究染色时间和浓度的作用。
结果
确定采用相对高浓度、短染色时间方法的油橄榄果油浴可产生适合创建精确三维渲染的高质量图像。
结论
使用碘染色技术对流体中的心脏瓣膜进行微型计算机断层扫描被证明是可行的。
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