Department of Physics, Air University, PAF Complex, E-9, Islamabad 44000, Pakistan.
Department of Animal Sciences, Quaid e Azam University, Islamabad 44000, Pakistan.
Biomed Res Int. 2021 Jul 15;2021:9916909. doi: 10.1155/2021/9916909. eCollection 2021.
The fabrication of sensitive protein microarrays such as PCR used in DNA microarray is challenging due to lack of signal amplification. The development of microarrays is utilized to improve the sensitivity and limitations of detection towards primal cancer detection. The sensitivity is enhanced by the use of ZnO-nanorods and is investigated as a substrate which enhance the florescent signal to diagnose the hepatocellular carcinoma (HCC) at early stages. The substrate for deposition of ZnO-nanorods is prepared by the conventional chemical bath deposition method. The resultant highly dense ZnO-nanorods enhance the fluorescent signal 7.2 times as compared to the substrate without ZnO-nanorods. The microarray showed sensitivity of 1504.7 ng ml and limit of detection of 0.1 pg ml in wide dynamic range of 0.05 pg-10 g ml for alpha fetoprotein (AFP) detection in 10% human serum. This immunoassay was successfully applied for human serum samples to detect tumor marker with good recoveries. The ZnO-nanorod substrate is a simple protein microarray which showed a great promise for developing a low-cost, sensitive, and high-throughput protein assay platform for several applications in both fundamental research and clinical diagnosis.
由于缺乏信号放大，敏感蛋白质微阵列（如用于 DNA 微阵列的 PCR）的制造具有挑战性。微阵列的发展被用于提高针对原发性癌症检测的灵敏度和检测限制。通过使用 ZnO 纳米棒来提高灵敏度，并将其作为一种基底来增强荧光信号，以在早期诊断肝细胞癌 (HCC)。通过传统的化学浴沉积方法制备 ZnO 纳米棒沉积的基底。与没有 ZnO 纳米棒的基底相比，高浓度的 ZnO 纳米棒将荧光信号增强了 7.2 倍。微阵列在宽动态范围内显示出对甲胎蛋白 (AFP) 的检测灵敏度为 1504.7 ng/ml，检测限为 0.1 pg/ml，在 10%人血清中检测 AFP 的浓度范围为 0.05 pg-10 g/ml。该免疫分析成功地应用于人血清样品中，以良好的回收率检测肿瘤标志物。ZnO 纳米棒基底是一种简单的蛋白质微阵列，为开发低成本、高灵敏度、高通量的蛋白质分析平台提供了很大的希望，可用于基础研究和临床诊断中的多种应用。
