School of Chemical and Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, Singapore 637457, Singapore.
Biosens Bioelectron. 2011 Apr 15;26(8):3683-7. doi: 10.1016/j.bios.2011.01.045. Epub 2011 Feb 26.
Current high-throughput antibody microarrays greatly rely on enzymatic reactions- or nanostructured tags-based reporter-amplifications for required sensitivity, but they need tedious operation, additional expensive reagents and microwells or microchannels to eliminate crossover interferences, thus resulting in low array density and high expense. A unique ZnO nanorods-grown substrate is developed here to not only immobilize a large amount of probe molecules, but also directly amplify the microarray fluorescent signals in detection of two important cancer biomarkers, carcinoembryonic antigen (CEA) and α-fetoprotein (AFP), achieving a detection limit of 1 pg mL(-1) in human serum, which is comparative to or lower than that of ELISA. This advanced ZnO nanorods-based substrate can be mass-manufactured, which offers great potential to fabricate economical and sensitive protein arrays for broad applications in clinic diagnosis, therapeutic monitoring and drug discovery.
目前高通量抗体微阵列在很大程度上依赖于基于酶反应或纳米结构标记的报告物放大来提高所需的灵敏度，但它们需要繁琐的操作、额外昂贵的试剂和微孔或微通道来消除交叉干扰，从而导致阵列密度低且费用高。在这里开发了一种独特的 ZnO 纳米棒生长基底，不仅可以固定大量的探针分子，而且还可以在检测两种重要的癌症生物标志物癌胚抗原（CEA）和甲胎蛋白（AFP）时直接放大微阵列荧光信号，在人血清中的检测限达到 1 pg mL(-1)，与 ELISA 相当或更低。这种先进的基于 ZnO 纳米棒的基底可以进行大规模制造，为在临床诊断、治疗监测和药物发现等广泛领域制造经济且灵敏的蛋白质阵列提供了巨大的潜力。
