Keuppens F, Willems G, Vansteenkiste Y, Woussen-Colle M C
Surg Gynecol Obstet. 1978 Mar;146(3):400-6.
The total number of gastric and duodenal gastrin cells was determined in the gastrectomy specimens from eight patients with peptic ulcer. Planimetry was used to determine the antral and duodenal surface. The immunoperoxydase method with specific antigastrin antibodies was used for staining gastrin cells, and the mean concentration of nucleated gastrin cells per square millimeter of antral and duodenal surface was determined by light microscopy. The mean number of duodenal gastrin cells in the resected duodenum was 4.8 per cent of the total gastric gastrin cell mass. The concentration of gastrin cells in the antrum was quite variable from one mucosal site to another. The degree of extension of the antral gastritic areas was a major factor influencing the mean concentration value. The total number of gastrin cells in the stomach varied from approximately 7 to 74 million cells. Our data indicate that hyperplasia of gastrin cells cannot be demonstrated by studies performed in small specimens taken for biopsy.
对8例消化性溃疡患者胃切除标本中的胃和十二指肠胃泌素细胞总数进行了测定。采用面积测量法测定胃窦和十二指肠表面面积。用抗胃泌素特异性抗体的免疫过氧化物酶法对胃泌素细胞进行染色，并通过光学显微镜测定每平方毫米胃窦和十二指肠表面有核胃泌素细胞的平均浓度。切除的十二指肠中十二指肠胃泌素细胞的平均数量占胃胃泌素细胞总量的4.8%。胃窦中胃泌素细胞的浓度在不同黏膜部位差异很大。胃窦胃炎区域的扩展程度是影响平均浓度值的主要因素。胃中胃泌素细胞总数约为700万至7400万个。我们的数据表明，通过活检获取的小标本研究无法证实胃泌素细胞增生。
