State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun, Jilin, 130022, PR China; University of Science and Technology of China, Hefei, Anhui, 230026, PR China.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun, Jilin, 130022, PR China.
Biosens Bioelectron. 2021 Nov 15;192:113532. doi: 10.1016/j.bios.2021.113532. Epub 2021 Jul 24.
Herein, the aggregation-induced electrochemiluminescence (AIECL) of a distyrylarylene derivative, 4,4'-bis(2,2-diphenylvinyl)-1,1'-biphenyl (DPVBi), was investigated for the first time. This luminophore exhibits significantly enhanced photoluminescence (PL) and electrochemiluminescence (ECL) emission with the increases of water content in organic/water mixtures. This high luminescence efficiency of DPVBi in aggregate state is due to the fact that the aggregates can reduce the energy loss by restricting the intramolecular motions. The ECL behavior of DPVBi in acetonitrile was investigated by ECL transients and so-called "half-scan" technology, where singlet-singlet annihilation ECL was generated under continuous potential switching. The DPVBi nanobulks (DPVBi NBs) were prepared to improve its application in aqueous media, which could be conveniently cast on electrode surface for developing sensing platform due to its good film-forming nature. The constructed heterogeneous AIECL platform can produce reductive-oxidative and oxidative-reductive ECL by using trimethylamine (TEA) and potassium peroxodisulfate (KSO) as coreactant. On the basis of the higher ECL efficiency of DPVBi NBs/TEA system, a label free immunosensor for cardiac troponin I (cTnI) was developed with the assistance of electrodeposited gold nanoparticles, and it showed a wide linear range of 20 ng/mL~100 fg/mL and low detection limit of 43 fg/mL. Moreover, the constructed immunosensor also exhibited good specificity, stability and satisfied performance in practical sample analysis.
本文首次研究了二苯乙烯芳基衍生物 4,4'-双(2,2-二苯基乙烯基)-1,1'-联苯(DPVBi)的聚集诱导电化学发光(AIECL)。该发光体在有机/水混合物中随着含水量的增加，表现出显著增强的光致发光(PL)和电化学发光(ECL)发射。DPVBi 在聚集态下具有如此高的发光效率，是因为聚集态可以通过限制分子内运动来减少能量损失。通过 ECL 瞬态和所谓的“半扫描”技术研究了 DPVBi 在乙腈中的 ECL 行为，其中在连续电位切换下产生了单重态-单重态湮灭 ECL。为了提高其在水相中的应用，制备了 DPVBi 纳米块(DPVBi NBs)，由于其良好的成膜性，可方便地浇铸在电极表面，用于开发传感平台。所构建的非均相 AIECL 平台可通过使用三甲胺(TEA)和过硫酸钾(KSO)作为共反应物产生还原-氧化和氧化-还原 ECL。基于 DPVBi NBs/TEA 体系具有更高的 ECL 效率，在电沉积金纳米粒子的辅助下，开发了一种用于检测心肌肌钙蛋白 I(cTnI)的无标记免疫传感器，其线性范围为 20ng/mL~100fg/mL，检测限低至 43fg/mL。此外，所构建的免疫传感器在实际样品分析中还表现出良好的特异性、稳定性和令人满意的性能。
