Ozden A, Degertekin H, Yeginsu A O, Ertan A
Dept. of Medicine, Tulane University Medical School, New Orleans, LA 70112.
Scand J Gastroenterol. 1987 Dec;22(10):1257-62. doi: 10.3109/00365528708996473.
We studied in five healthy volunteers whether the cholinergic pathway regulated the secretion of gastric intraluminal somatostatin-like immunoreactivity (SLI) in response to stimuli of pentagastrin infusion (0.9 micrograms/kg/h, intravenously) and sham feeding. We measured gastric secretory volume, hydrogen ion output, and SLI at base line, during pentagastrin infusion, after sham feeding, and after applications of atropine (0.0, 0.7, 7.0 micrograms/kg, intramuscularly) given before pentagastrin and sham feeding. The stimuli were given randomly, at separate times on different days. After each stimulus, eight 15-min gastric juice collections were made; samples were adjusted to pH 7, pepstatin-A and aprotinin were added, and samples were extracted with acetone to determine SLI by radioimmunoassay. Pentagastrin and sham feeding significantly increased gastric luminal SLI secretion, which appeared to correlate with the increases in volume and acid output. Atropine at 7 micrograms/kg significantly suppressed gastric volume, acid, and SLI outputs stimulated by sham feeding; however, responses to pentagastrin stimulation remained unchanged. To conclude, the cholinergic mechanism regulates gastric intraluminal SLI response to sham feeding but not to pentagastrin infusion.
我们在五名健康志愿者中进行了研究，以探讨胆碱能途径是否调节胃腔内生长抑素样免疫反应性（SLI）的分泌，该分泌是对五肽胃泌素输注（0.9微克/千克/小时，静脉注射）和假饲刺激的反应。我们在基线、五肽胃泌素输注期间、假饲后以及在五肽胃泌素和假饲前肌肉注射阿托品（0.0、0.7、7.0微克/千克）后，测量了胃分泌量、氢离子输出量和SLI。刺激是随机给予的，在不同日期的不同时间进行。每次刺激后，收集八次15分钟的胃液；将样品调节至pH 7，加入胃蛋白酶抑制剂A和抑肽酶，并用丙酮提取样品，通过放射免疫测定法测定SLI。五肽胃泌素和假饲显著增加了胃腔内SLI的分泌，这似乎与体积和酸输出的增加相关。7微克/千克的阿托品显著抑制了假饲刺激的胃体积、酸和SLI输出；然而，对五肽胃泌素刺激的反应保持不变。总之，胆碱能机制调节胃腔内SLI对假饲的反应，但不调节对五肽胃泌素输注的反应。
