用于诺如病毒感染诊断的环介导等温扩增检测法的汇总分析。

Pooled analysis of LAMP assay for the diagnosis of norovirus infection.

机构信息

Department of Clinical Laboratory Medicine, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

Department of Clinical Medicine, The Sixth Clinical School of Guangzhou Medical University, Guangzhou, China.

出版信息

J Clin Lab Anal. 2021 Sep;35(9):e23919. doi: 10.1002/jcla.23919. Epub 2021 Jul 31.

DOI:10.1002/jcla.23919

PMID:34331319

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8418469/

Abstract

BACKGROUND

Rapid laboratory detection is essential to diagnose norovirus infection. LAMP has many advantages compared with RT-PCR for detecting norovirus, including high sensitivity, high specificity, rapidity, low cost, and intuitive results, which can be easily read with the naked eye with the help of color-based reporters. In this study, we intend to analyze the accuracy of LAMP methods for the diagnosis of norovirus infection.

METHODS

Two researchers independently retrieved relevant literature up to January 2021 (PubMed, Web of Science, Cochrane Library, Embase, CNKI, Wan Fang, and VIP). The researchers screened all articles and extracted their research data for meta-analysis. QUADAS-2 tool was used to evaluate the quality of the included studies by Review Manager 5.3. Forest plots were performed by Meta-DiSc 1.4 to evaluate the accuracy of the test. Deeks' funnel plot symmetry tests were conducted by Stata 15.0 to check the potential publication bias.

RESULTS

Eleven sets of data extracted from the eight included studies were included for meta-analysis. For the detection of norovirus, the pooled sensitivity, specificity, positive LR, negative LR, diagnostic OR, and their 95% CI were 0.96 (0.95-0.97), 0.99 (0.99-1.00), 91.14 (31.88-260.56), 0.06 (0.04-0.09), and 1473.68 (562.96-3857.70), respectively. Besides, AUC in the SROC curve was 0.9920.

CONCLUSION

LAMP had high sensitivity and specificity in terms of the diagnosis of norovirus infection. However, further extension of this approach should be researched to ensure the accuracy and practicability of this hopeful test in the future.

摘要

背景

快速的实验室检测对于诊断诺如病毒感染至关重要。与 RT-PCR 相比，LAMP 具有许多优势，包括高灵敏度、高特异性、快速、低成本和直观的结果，借助基于颜色的报告器可以很容易地用肉眼读取。本研究旨在分析 LAMP 方法诊断诺如病毒感染的准确性。

方法

两位研究人员独立检索了截至 2021 年 1 月的相关文献（PubMed、Web of Science、Cochrane Library、Embase、CNKI、万方和 VIP）。研究人员筛选了所有文章并提取了他们的研究数据进行荟萃分析。使用 Review Manager 5.3 中的 QUADAS-2 工具评估纳入研究的质量。通过 Meta-DiSc 1.4 进行森林图分析以评估检验的准确性。使用 Stata 15.0 进行 Deeks' 漏斗图对称性检验以检查潜在的发表偏倚。

结果

从 8 项纳入的研究中提取了 11 组数据进行荟萃分析。用于检测诺如病毒时，汇总的敏感性、特异性、阳性似然比、阴性似然比、诊断比值比及其 95%CI 分别为 0.96（0.95-0.97）、0.99（0.99-1.00）、91.14（31.88-260.56）、0.06（0.04-0.09）和 1473.68（562.96-3857.70）。此外，SROC 曲线中的 AUC 为 0.9920。