Department of Clinical Laboratory Medicine, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, China.
Department of Clinical Medicine, the Third Clinical School of Guangzhou Medical University, Guangzhou, 511436, China.
BMC Infect Dis. 2020 Jul 20;20(1):525. doi: 10.1186/s12879-020-05250-w.
Neisseria meningitidis is a major cause of bacterial meningitis, and these infections are associated with a high mortality rate. Rapid and reliable diagnosis of bacterial meningitis is critical in clinical practice. However, this disease often occurs in economically depressed areas, so an inexpensive, easy to use, and accurate technology is needed. We performed a pooled-analysis to assess the potential of the recently developed loop-mediated isothermal amplification (LAMP) assay for detection of meningococcus.
Pubmed, Embase, and Web of Science were searched to identify original studies that used the LAMP assay to detect meningococcus. After pooling of data, the sensitivity and specificity were calculated, a summary receiver operating characteristic (SROC) curve was determined, and the area under the SROC curve was computed to determine diagnostic accuracy. Publication bias was assessed using Deek's funnel plot.
We examined 14 studies within 6 publications. The LAMP assay had high sensitivity (94%) and specificity (100%) in the detection of meningococcus in all studies. The area under the SROC curve (0.980) indicated high overall accuracy of the LAMP assay. There was no evidence of publication bias.
The LAMP assay has accuracy comparable to bacterial culture and PCR for detection of meningococcus, but is less expensive and easier to use. We suggest the adoption of the LAMP assay to detect meningococcus, especially in economically depressed areas.
脑膜炎奈瑟菌是细菌性脑膜炎的主要病因，此类感染与高死亡率相关。在临床实践中，快速且可靠的细菌性脑膜炎诊断至关重要。然而，这种疾病常发生在经济不发达地区，因此需要一种廉价、易用且准确的技术。我们进行了汇总分析，以评估新开发的环介导等温扩增（LAMP）检测方法对脑膜炎奈瑟菌检测的潜力。
通过检索 Pubmed、Embase 和 Web of Science，确定了使用 LAMP 检测方法检测脑膜炎奈瑟菌的原始研究。汇总数据后，计算了敏感性和特异性，绘制了汇总受试者工作特征（SROC）曲线，并计算了 SROC 曲线下面积以确定诊断准确性。采用 Deek's 漏斗图评估发表偏倚。
我们在 6 篇文献中检查了 14 项研究。在所有研究中，LAMP 检测方法对脑膜炎奈瑟菌的检测具有高敏感性（94%）和特异性（100%）。SROC 曲线下面积（0.980）表明 LAMP 检测方法具有较高的总体准确性。未发现发表偏倚的证据。
LAMP 检测方法在检测脑膜炎奈瑟菌方面的准确性与细菌培养和 PCR 相当，但成本更低，使用更方便。我们建议采用 LAMP 检测方法来检测脑膜炎奈瑟菌，特别是在经济不发达地区。
