Department of Biomedical Sciences, Faculty of Science, University of Ngaoundere, Cameroun.
Anato-Cytopathology Laboratory, Centre Hospitalier Dominicain Saint Martin de Porres, Yaounde, Cameroun; Cameroon Evangelical University Institute, Bandjoun, Cameroun.
Int J Infect Dis. 2021 Sep;110:426-432. doi: 10.1016/j.ijid.2021.07.059. Epub 2021 Jul 29.
To characterize high-risk HPV types associated with cervical precancerous lesions in women living in Yaounde, Cameroon, and to determine their distribution with HIV status.
Women with abnormal pap smears recorded from February 2015 to May 2019 at Saint Martin de Porres' Health Centre, Yaounde, Cameroon, were recruited in this study after obtaining informed consent. Pap smears were collected and re-examined. Human immunodeficiency virus (HIV) serology was determined. HPV16, 18, 33, and 45 were assessed using standard PCR.
All included participants (370) were HPV-positive and had either low grade squamous intraepithelial lesions (67.03%) or high grade squamous intraepithelial lesions (31.35%). They were subdivided into HIV-positive (N =102) and HIV-negative (N =268). In the HIV-negative subgroup, we observed 66.04% HPV16-positve, 41.79% HPV18-positve, 21.27% HPV33-positve and 8.21% HPV45-positve. In the HIV-positive subgroup, we observed 22.55% HPV16-positve, 5.88% HPV18-positve, 75.49% HPV33-positve, and 49.02% HPV45-positve. Married HIV-positive participants (47.14 ± 1.19) were older than both their single counterparts (34.94±1.22, P = 0.0008) and HIV-negative participants (41.43 ± 0.79, P = 0.0001). Single HIV-positive women reported higher numbers of miscarriages (P = 0.0023), and had later first sexual intercourse than HIV-negative (P = 0.0079) women.
Our study suggested differential expressions in high-risk HPV types with HIV status and cervical precancerous lesions and warrants more extensive studies.
描述喀麦隆雅温得高危型人乳头瘤病毒（HPV）与宫颈癌前病变相关的类型，并确定其与人类免疫缺陷病毒（HIV）感染状态的分布情况。
本研究于 2015 年 2 月至 2019 年 5 月在喀麦隆雅温得圣马丁德波雷医疗中心收集异常巴氏涂片的女性，在获得知情同意后将其纳入研究。采集巴氏涂片并进行复查，检测 HIV 血清学。采用标准聚合酶链反应（PCR）检测 HPV16、18、33 和 45。
所有纳入的 370 名参与者均为 HPV 阳性，其中低级别鳞状上皮内病变（LSIL）占 67.03%，高级别鳞状上皮内病变（HSIL）占 31.35%。他们被分为 HIV 阳性（N=102）和 HIV 阴性（N=268）两组。在 HIV 阴性亚组中，我们观察到 66.04%的 HPV16 阳性、41.79%的 HPV18 阳性、21.27%的 HPV33 阳性和 8.21%的 HPV45 阳性。在 HIV 阳性亚组中，我们观察到 22.55%的 HPV16 阳性、5.88%的 HPV18 阳性、75.49%的 HPV33 阳性和 49.02%的 HPV45 阳性。已婚的 HIV 阳性参与者（47.14±1.19）比单身的参与者（34.94±1.22，P=0.0008）和 HIV 阴性的参与者（41.43±0.79，P=0.0001）年龄更大。单身的 HIV 阳性女性报告的流产次数更多（P=0.0023），并且首次性行为的时间晚于 HIV 阴性女性（P=0.0079）。
我们的研究表明，高危型 HPV 类型的表达与 HIV 状态和宫颈癌前病变存在差异，需要进行更广泛的研究。
