College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China.
College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China; Institute of Natural Resources and Ecology Heilongjiang Academy of Sciences, Harbin 150040, China.
Bioresour Technol. 2021 Nov;339:125515. doi: 10.1016/j.biortech.2021.125515. Epub 2021 Jul 8.
In this study, influence of biochar on nitrification was explored using multi-level (DNA, RNA, protein) and multi-aspect (diversity, structure, key community, co-occurrence pattern and functional modules) analyses (M-LAA) of ammonia-oxidizing microorganisms (AOMs) during cattle manure composting. Biochar addition increased the copy numbers and diversity of AOMs, restricted (36.02%) the amoA gene transcripts of archaea but increased (24.53%) those of bacteria, and reduced (75.86%) ammonooxygenase (AMO) activity. Crenarchaeota and Thaumarcheota mediated NH-N, Unclassified_k_norank_d_Archaea and Crenarchaeota regulated AMO activity and potential ammonia oxidation (PAO) rates. Nitrosomonas and Nitrosospira were the predominant microbial taxa influencing NH-N variation and PAO rates, respectively. Additionally, both Crenarchaeota and Nitrosospira played crucial roles in mediating NO-N and NO-N. Furthermore, biochar altered the network patterns of AOMs community by changing the keystone species and the interactivity among communities. These findings indicated that influence of biochar on nitrification could be better explained using M-LAA of AOMs.
在这项研究中，使用氨氧化微生物（AOM）的多层次（DNA、RNA、蛋白质）和多方面（多样性、结构、关键群落、共生模式和功能模块）分析（M-LAA）来探索生物炭对硝化作用的影响，研究了牛粪堆肥过程中生物炭的添加对氨氧化微生物（AOM）的影响。生物炭的添加增加了 AOM 的拷贝数和多样性，限制了古菌amoA 基因转录（36.02%），但增加了细菌amoA 基因转录（24.53%），并降低了氨氧化酶（AMO）活性（75.86%）。Crenarchaeota 和 Thaumarcheota 介导 NH-N，未分类的_k_norank_d_古菌和 Crenarchaeota 调节 AMO 活性和潜在氨氧化（PAO）速率。Nitrosomonas 和 Nitrosospira 分别是影响 NH-N 变化和 PAO 速率的主要微生物类群。此外，Crenarchaeota 和 Nitrosospira 在介导 NO-N 和 NO-N 方面都起着至关重要的作用。此外，生物炭通过改变关键物种和群落间的相互作用改变了 AOM 群落的网络模式。这些发现表明，生物炭对硝化作用的影响可以通过 AOM 的 M-LAA 更好地解释。
