Morozova E O, Mamaev N N, Blinov M N, Samuskevich I G, Medvedeva N V
Tsitologiia. 1987 Oct;29(10):1150-5.
A study was made of the number of silver grains over the nucleoli and of the content of ribosomes in the lymphocyte cytoplasm for six healthy persons and for 20 patients with chronic lymphatic leukemia. Besides ratios of compact, nucleolonemic and ring types of nucleoli were evaluated in addition to counts of the specific radioactivity of mature 28S rRNA in lymphocytes. In the majority of cases examined, cells with 1 or 2 nucleoli of compact and nucleolonemic types were seen dominating. The number of silver grains over the nucleoli in the control healthy persons did not differ from that in patients who did not receive any treatment, which contrasted with high value grain counts in the treated patients. The lymphocyte ribosome contents varied within the normal and decreased values in both the patient groups. The specific radioactivity in 28S rRNA leukemic lymphocytes was significantly lower in groups of patients with low ribosome contents than in those with the normal ones. The data suggest that in the leukemic cells with a high or unaltered activity of ribosome cistrons and low ribosome levels rRNA processing is broken.
对6名健康人和20名慢性淋巴细胞白血病患者的核仁上银颗粒数量以及淋巴细胞胞质中核糖体含量进行了研究。除了评估致密型、核仁缢痕型和环状核仁的比例外，还对淋巴细胞中成熟28S rRNA的比放射性进行了计数。在大多数检查的病例中，可见具有1个或2个致密型和核仁缢痕型核仁的细胞占主导。未接受任何治疗的患者核仁上的银颗粒数量与对照健康人无差异，这与接受治疗的患者银颗粒计数高形成对比。两组患者的淋巴细胞核糖体含量在正常范围内变化且均有降低。核糖体含量低的患者组中白血病淋巴细胞28S rRNA的比放射性明显低于核糖体含量正常的患者组。数据表明，在核糖体顺反子活性高或未改变但核糖体水平低的白血病细胞中，rRNA加工被破坏。
