Mei Chenxue, Wang Xiao, Meng Fanxiang, Zhang Xiaoqing, Chen Ling, Yan Siqi, Xue Junxiu, Sun Xun, Wang Yuanyuan
Department of Immunology, College of Basic Medical Sciences of China Medical University, Shenyang, China.
Department of Gastroenterology, Shengjing Hospital of China Medical University, Shenyang, China.
Front Pharmacol. 2021 Jul 16;12:696599. doi: 10.3389/fphar.2021.696599. eCollection 2021.
Aucuboside is an iridoid glycoside extracted from traditional Chinese medicine such as , possessing a wide range of biological activities, including antioxidant, anti-aging, anti-inflammatory, and anti-fibrotic effects. The effects of aucuboside on inflammatory bowel disease (IBD) have not been studied. Therefore, the effects of aucuboside on the generation of Foxp3+ regulatory T (Treg) cells and IL-17-producing T helper (Th17) cells in colitis were studied. A mouse colitis model was established by intracolonic administration of 2,4,6-trinitrobenzene sulfonic acid (TNBS) to mimic human IBD. The generation of Treg and Th17 cells was evaluated by flow cytometry. Aucuboside significantly alleviated colitis symptoms, including weight loss, high disease activity index, and inflammatory responses. The generation of Th17 cells in colitis was significantly inhibited by aucuboside and accompanied by the suppression of IL-17 expression. In Raw264.7 cells, the LPS-induced increase in IL-17 expression was also suppressed by aucuboside, which was significantly blocked by the RORγt inhibitor sr2211. In addition, the decrease in the proportion of Treg cells was also partially reversed by aucuboside, which may reflect the aucuboside-induced inhibition of Th17 cells. This previously unrecognized immunoregulatory function of aucuboside may have clinical applications in IBD.
桃叶珊瑚苷是从诸如等中药中提取的一种环烯醚萜苷，具有广泛的生物活性，包括抗氧化、抗衰老、抗炎和抗纤维化作用。桃叶珊瑚苷对炎症性肠病（IBD）的影响尚未得到研究。因此，研究了桃叶珊瑚苷对结肠炎中Foxp3 +调节性T（Treg）细胞和产生白细胞介素-17的辅助性T（Th17）细胞生成的影响。通过向结肠内注射2,4,6-三硝基苯磺酸（TNBS）建立小鼠结肠炎模型以模拟人类IBD。通过流式细胞术评估Treg和Th17细胞的生成。桃叶珊瑚苷显著减轻了结肠炎症状，包括体重减轻、高疾病活动指数和炎症反应。桃叶珊瑚苷显著抑制了结肠炎中Th17细胞的生成，并伴随着白细胞介素-17表达的抑制。在Raw264.7细胞中，桃叶珊瑚苷也抑制了脂多糖诱导的白细胞介素-17表达增加，这被RORγt抑制剂sr2211显著阻断。此外，桃叶珊瑚苷还部分逆转了Treg细胞比例的下降，这可能反映了桃叶珊瑚苷对Th17细胞的诱导抑制作用。桃叶珊瑚苷这种以前未被认识的免疫调节功能可能在IBD中有临床应用。
