腰果在结肠炎小鼠模型中的抗氧化和抗炎特性。
The Antioxidant and Anti-Inflammatory Properties of L. Cashew Nuts in a Mouse Model of Colitis.
机构信息
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98166 Messina, Italy.
Department of Biomedical, Dental and Morphological and Functional Imaging University of Messina, Via Consolare Valeria, 98125 Messina, Italy.
出版信息
Nutrients. 2020 Mar 20;12(3):834. doi: 10.3390/nu12030834.
BACKGROUND
L. is a tropical plant used for the treatment of inflammatory diseases. The goal of the present work was to investigate the anti-inflammatory and anti-oxidant potential of oral administration of cashew nuts (from L.) in a mouse model of colitis.
METHODS
Induction of colitis was performed by intrarectally injection of dinitrobenzene sulfonic acid (DNBS). Cashew nuts were administered daily orally (100 mg/kg) in DNBS-injected mice.
RESULTS
Four days after DNBS, histological and macroscopic colon alterations as well as marked clinical signs and increased cytokine production were observed. Neutrophil infiltration, measured by myeloperoxidase (MPO) positive immunostaining, was correlated with up-regulation of adhesion molecules ICAM-1 and P-selectin in colons. Oxidative stress was detected with increased malondialdehyde (MDA) levels, nitrotyrosine, and poly ADP-ribose polymerase (PARP) positive staining in inflamed colons. Oral treatment with cashew nuts reduced histological, macroscopic damage, neutrophil infiltration, pro-inflammatory cytokines and MDA levels, as well as nitrotyrosine, PARP and ICAM-1, and P-selectin expressions. Colon inflammation could be related to nuclear factor (NF)-kB pathway activation and reduced manganese superoxide dismutase (MnSOD) antioxidant activity. Cashew nuts administration inhibited NF-kB and increased MnSOD antioxidant expressions.
CONCLUSIONS
The results suggested that oral assumption of cashew nuts may be beneficial for the management of colitis.
背景
腰果是一种热带植物，用于治疗炎症性疾病。本工作的目的是研究口服腰果（来自 L.）在结肠炎小鼠模型中的抗炎和抗氧化潜力。
方法
通过直肠内注射二硝基苯磺酸（DNBS）诱导结肠炎。在 DNBS 注射的小鼠中，每天口服给予腰果（100mg/kg）。
结果
在 DNBS 后 4 天，观察到组织学和宏观结肠改变以及明显的临床症状和细胞因子产生增加。髓过氧化物酶（MPO）阳性免疫染色测量的中性粒细胞浸润与结肠中粘附分子 ICAM-1 和 P-选择素的上调相关。氧化应激通过增加丙二醛（MDA）水平、硝基酪氨酸和多聚 ADP-核糖聚合酶（PARP）在炎症结肠中的阳性染色来检测。口服腰果治疗可减轻组织学、宏观损伤、中性粒细胞浸润、促炎细胞因子和 MDA 水平，以及硝基酪氨酸、PARP 和 ICAM-1 和 P-选择素的表达。结肠炎症可能与核因子（NF）-kB 途径的激活和锰超氧化物歧化酶（MnSOD）抗氧化活性的降低有关。腰果的给药抑制了 NF-kB 并增加了 MnSOD 抗氧化表达。
结论
结果表明，口服腰果可能有益于结肠炎的治疗。
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