Li Changfu, Pan Damin, Li Mengyuan, Wang Yao, Song Luting, Yu Danyang, Zuo Yuxin, Wang Kenan, Liu Yuqi, Wei Zhiyan, Lu Zhiqiang, Zhu Lingfang, Shen Xihui
State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling, China.
Department of Entomology, College of Plant Protection, Northwest A&F University, Yangling, China.
Front Microbiol. 2021 Jul 15;12:699913. doi: 10.3389/fmicb.2021.699913. eCollection 2021.
Aerobactin is a citrate-hydroxamate siderophore that is critical for the virulence of pathogenic enteric bacteria. However, although the aerobactin-producing - operon is distributed widely in the genomes of species, none of the pathogenic spp. was found to produce aerobactin. Here, we showed that the - operon in the food-borne enteric pathogen YPIII is a functional siderophore system involved in iron acquisition. The expression of the operon was found to be directly repressed by the ferric uptake regulator (Fur) in an iron concentration-dependent manner. In addition, we demonstrated that the aerobactin-mediated iron acquisition contributes to bacterial growth under iron-limited conditions. Moreover, we provided evidence that aerobactin plays important roles in biofilm formation, resistance to oxidative stress, ROS removal, and virulence of . Overall, our study not only uncovered a novel strategy of iron acquisition in but also highlighted the importance of aerobactin in the pathogenesis of .
气杆菌素是一种柠檬酸异羟肟酸型铁载体，对致病性肠道细菌的毒力至关重要。然而，尽管产气杆菌素的操纵子广泛分布于多种物种的基因组中，但未发现任何致病性[具体物种]产生气杆菌素。在此，我们表明食源肠道病原体[具体病原体名称]YPIII中的[具体操纵子名称]操纵子是一个参与铁获取的功能性铁载体系统。发现该操纵子的表达受到铁摄取调节蛋白（Fur）以铁浓度依赖性方式的直接抑制。此外，我们证明气杆菌素介导的铁获取有助于细菌在铁限制条件下的生长。而且，我们提供的证据表明气杆菌素在[具体病原体名称]的生物膜形成、抗氧化应激、活性氧清除和毒力方面发挥重要作用。总体而言，我们的研究不仅揭示了[具体病原体名称]中铁获取的新策略，还突出了气杆菌素在[具体病原体名称]发病机制中的重要性。
